Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2188061..2188647 Replicon chromosome
Accession NC_018078
Organism Mycobacterium tuberculosis KZN 605

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag TBXG_RS10445 Protein ID WP_003410010.1
Coordinates 2188303..2188647 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag TBXG_RS10440 Protein ID WP_003410014.1
Coordinates 2188061..2188309 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBXG_RS10420 2184044..2184811 - 768 WP_003410019.1 hemerythrin domain-containing protein -
TBXG_RS10425 2184968..2185324 + 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
TBXG_RS10430 2185377..2185649 + 273 WP_003410017.1 DUF1490 family protein -
TBXG_RS10435 2185646..2187961 + 2316 WP_003899120.1 cation transporter ATPase CptG -
TBXG_RS10440 2188061..2188309 + 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
TBXG_RS10445 2188303..2188647 + 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
TBXG_RS10450 2188736..2189071 + 336 WP_003410009.1 dehydrogenase -
TBXG_RS10455 2188972..2189229 - 258 WP_003410006.1 hypothetical protein -
TBXG_RS10460 2189315..2189656 + 342 WP_003410003.1 DUF2384 domain-containing protein -
TBXG_RS10465 2189653..2190213 + 561 WP_003410001.1 RES family NAD+ phosphorylase -
TBXG_RS10470 2190733..2191272 - 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
TBXG_RS10475 2191498..2191926 - 429 WP_003906713.1 cellulose-binding protein -
TBXG_RS10480 2192342..2192941 - 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T27974 WP_003410010.1 NC_018078:2188303-2188647 [Mycobacterium tuberculosis KZN 605]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T27974 NC_018078:2188303-2188647 [Mycobacterium tuberculosis KZN 605]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT27974 WP_003410014.1 NC_018078:2188061-2188309 [Mycobacterium tuberculosis KZN 605]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT27974 NC_018078:2188061-2188309 [Mycobacterium tuberculosis KZN 605]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References