Detailed information of TA system TA027973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2164281..2164907
• Replicon: chromosome
• Accession: NC_018078
• Organism: Mycobacterium tuberculosis KZN 605

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: TBXG_RS10340
• Protein ID: WP_003410075.1
• Coordinates: 2164281..2164679 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: TBXG_RS10345
• Protein ID: WP_003911750.1
• Coordinates: 2164680..2164907 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBXG_RS10315	2159880..2161136	+	1257	WP_003410095.1	HNH endonuclease	-
TBXG_RS10320	2161264..2161854	-	591	WP_003899131.1	IS110 family transposase	-
TBXG_RS10325	2161808..2162455	-	648	WP_071854215.1	transposase	-
TBXG_RS21265	2162516..2162695	+	180	Protein_2017	hypothetical protein	-
TBXG_RS10330	2163132..2163566	-	435	WP_003914358.1	DUF1398 domain-containing protein	-
TBXG_RS10335	2163667..2164098	+	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
TBXG_RS10340	2164281..2164679	-	399	WP_003410075.1	PIN domain nuclease	Toxin
TBXG_RS10345	2164680..2164907	-	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBXG_RS10350	2165166..2166335	+	1170	WP_003899126.1	ATP-binding protein	-
TBXG_RS10355	2166524..2166868	+	345	WP_003410065.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T27973 WP_003410075.1 NC_018078:c2164679-2164281 [Mycobacterium tuberculosis KZN 605]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T27973 NC_018078:c2164679-2164281 [Mycobacterium tuberculosis KZN 605]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT27973 WP_003911750.1 NC_018078:c2164907-2164680 [Mycobacterium tuberculosis KZN 605]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT27973 NC_018078:c2164907-2164680 [Mycobacterium tuberculosis KZN 605]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8