Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1543563..1544200 Replicon chromosome
Accession NC_018078
Organism Mycobacterium tuberculosis KZN 605

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag TBXG_RS07385 Protein ID WP_003413180.1
Coordinates 1543805..1544200 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag TBXG_RS07380 Protein ID WP_003413183.1
Coordinates 1543563..1543808 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBXG_RS07360 1540157..1540669 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
TBXG_RS07365 1540662..1541915 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
TBXG_RS07370 1541912..1542721 + 810 WP_003413193.1 shikimate dehydrogenase -
TBXG_RS07375 1542733..1543152 + 420 WP_003413190.1 A24 family peptidase -
TBXG_RS07380 1543563..1543808 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
TBXG_RS07385 1543805..1544200 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
TBXG_RS07390 1544300..1544674 + 375 WP_003413177.1 hypothetical protein -
TBXG_RS07395 1544690..1545067 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
TBXG_RS07400 1545064..1545321 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
TBXG_RS07405 1545360..1545773 - 414 WP_003413164.1 PIN domain nuclease -
TBXG_RS07410 1545866..1546144 - 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
TBXG_RS07415 1546141..1546803 - 663 WP_003900848.1 hypothetical protein -
TBXG_RS07420 1546800..1547459 - 660 WP_003900846.1 LppA family lipoprotein -
TBXG_RS07425 1547586..1548797 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T27964 WP_003413180.1 NC_018078:1543805-1544200 [Mycobacterium tuberculosis KZN 605]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T27964 NC_018078:1543805-1544200 [Mycobacterium tuberculosis KZN 605]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT27964 WP_003413183.1 NC_018078:1543563-1543808 [Mycobacterium tuberculosis KZN 605]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT27964 NC_018078:1543563-1543808 [Mycobacterium tuberculosis KZN 605]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References