Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4155686..4156262 Replicon chromosome
Accession NZ_CP124345
Organism Escherichia coli strain AVS0530

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP77_RS20275 Protein ID WP_001297643.1
Coordinates 4155975..4156262 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP77_RS20270 Protein ID WP_000063156.1
Coordinates 4155686..4155988 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP77_RS20255 (4152282) 4152282..4154432 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP77_RS20260 (4154482) 4154482..4154685 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP77_RS20265 (4154696) 4154696..4155652 + 957 WP_001297640.1 GTPase -
QJP77_RS20270 (4155686) 4155686..4155988 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP77_RS20275 (4155975) 4155975..4156262 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP77_RS20280 (4156461) 4156461..4157158 - 698 Protein_3980 IS1 family transposase -
QJP77_RS20285 (4157215) 4157215..4157310 + 96 Protein_3981 dienelactone hydrolase family protein -
QJP77_RS20290 (4157456) 4157456..4158688 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP77_RS20295 (4158729) 4158729..4160009 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T279639 WP_001297643.1 NZ_CP124345:c4156262-4155975 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT279639 WP_000063156.1 NZ_CP124345:c4155988-4155686 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References