Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4155810..4156386 Replicon chromosome
Accession NZ_CP124343
Organism Escherichia coli strain AVS0533

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP85_RS20290 Protein ID WP_001297643.1
Coordinates 4156099..4156386 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP85_RS20285 Protein ID WP_000063156.1
Coordinates 4155810..4156112 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP85_RS20270 (4152406) 4152406..4154556 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP85_RS20275 (4154606) 4154606..4154809 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP85_RS20280 (4154820) 4154820..4155776 + 957 WP_001297640.1 GTPase -
QJP85_RS20285 (4155810) 4155810..4156112 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP85_RS20290 (4156099) 4156099..4156386 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP85_RS20295 (4156585) 4156585..4157282 - 698 Protein_3980 IS1 family transposase -
QJP85_RS20300 (4157339) 4157339..4157434 + 96 Protein_3981 dienelactone hydrolase family protein -
QJP85_RS20305 (4157580) 4157580..4158812 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP85_RS20310 (4158853) 4158853..4160133 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T279617 WP_001297643.1 NZ_CP124343:c4156386-4156099 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT279617 WP_000063156.1 NZ_CP124343:c4156112-4155810 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References