Detailed information of TA system TA027959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1303032..1303602
• Replicon: chromosome
• Accession: NC_018078
• Organism: Mycobacterium tuberculosis KZN 605

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: TBXG_RS06080
• Protein ID: WP_003414166.1
• Coordinates: 1303246..1303602 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: TBXG_RS06075
• Protein ID: WP_003901465.1
• Coordinates: 1303032..1303262 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBXG_RS06035	1298050..1298361	-	312	WP_003414190.1	hypothetical protein	-
TBXG_RS06040	1298466..1298723	-	258	WP_003899489.1	hypothetical protein	-
TBXG_RS06045	1298957..1300246	-	1290	Protein_1188	transposase family protein	-
TBXG_RS06050	1300310..1300501	-	192	WP_003414184.1	hypothetical protein	-
TBXG_RS06055	1300498..1300902	-	405	WP_009938577.1	hypothetical protein	-
TBXG_RS22090	1301050..1301304	+	255	WP_003917684.1	hypothetical protein	-
TBXG_RS22095	1301480..1301755	-	276	WP_003911993.1	DUF1778 domain-containing protein	-
TBXG_RS06070	1301946..1302989	+	1044	WP_003414172.1	DUF2293 domain-containing protein	-
TBXG_RS06075	1303032..1303262	+	231	WP_003901465.1	antitoxin MazE	Antitoxin
TBXG_RS06080	1303246..1303602	+	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
TBXG_RS06085	1303704..1305353	-	1650	WP_003899485.1	CocE/NonD family hydrolase	-
TBXG_RS06090	1305372..1305959	-	588	WP_003914429.1	DUF3558 family protein	-
TBXG_RS06095	1306132..1306458	+	327	WP_003414157.1	hypothetical protein	-
TBXG_RS06100	1306462..1308150	+	1689	WP_003414155.1	alpha/beta hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1292393..1331492	39099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T27959 WP_003414166.1 NC_018078:1303246-1303602 [Mycobacterium tuberculosis KZN 605]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T27959 NC_018078:1303246-1303602 [Mycobacterium tuberculosis KZN 605]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT27959 WP_003901465.1 NC_018078:1303032-1303262 [Mycobacterium tuberculosis KZN 605]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT27959 NC_018078:1303032-1303262 [Mycobacterium tuberculosis KZN 605]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3