Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3770386..3770962 Replicon chromosome
Accession NZ_CP124332
Organism Escherichia coli strain AVS0753

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag QJP82_RS18560 Protein ID WP_001295743.1
Coordinates 3770386..3770673 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag QJP82_RS18565 Protein ID WP_000063148.1
Coordinates 3770660..3770962 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP82_RS18545 (3766046) 3766046..3767350 - 1305 WP_000535012.1 restriction endonuclease subunit S -
QJP82_RS18550 (3767340) 3767340..3768959 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
QJP82_RS18555 (3769023) 3769023..3770189 - 1167 WP_000800831.1 restriction endonuclease -
QJP82_RS18560 (3770386) 3770386..3770673 + 288 WP_001295743.1 BrnT family toxin Toxin
QJP82_RS18565 (3770660) 3770660..3770962 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
QJP82_RS18570 (3770996) 3770996..3771952 - 957 WP_001295745.1 GTPase -
QJP82_RS18575 (3771963) 3771963..3772166 - 204 WP_000467859.1 YbdD/YjiX family protein -
QJP82_RS18580 (3772216) 3772216..3774366 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T279494 WP_001295743.1 NZ_CP124332:3770386-3770673 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT279494 WP_000063148.1 NZ_CP124332:3770660-3770962 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References