Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4154136..4154712 Replicon chromosome
Accession NZ_CP124322
Organism Escherichia coli strain AVS0717

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJB10_RS20285 Protein ID WP_001297643.1
Coordinates 4154425..4154712 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJB10_RS20280 Protein ID WP_000063156.1
Coordinates 4154136..4154438 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJB10_RS20265 (4150732) 4150732..4152882 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJB10_RS20270 (4152932) 4152932..4153135 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJB10_RS20275 (4153146) 4153146..4154102 + 957 WP_001297640.1 GTPase -
QJB10_RS20280 (4154136) 4154136..4154438 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJB10_RS20285 (4154425) 4154425..4154712 - 288 WP_001297643.1 BrnT family toxin Toxin
QJB10_RS20290 (4154911) 4154911..4155608 - 698 Protein_3979 IS1 family transposase -
QJB10_RS20295 (4155665) 4155665..4155760 + 96 Protein_3980 dienelactone hydrolase family protein -
QJB10_RS20300 (4155906) 4155906..4157138 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJB10_RS20305 (4157179) 4157179..4158459 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T279389 WP_001297643.1 NZ_CP124322:c4154712-4154425 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT279389 WP_000063156.1 NZ_CP124322:c4154438-4154136 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References