Detailed information of TA system TA027935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3721765..3722330
• Replicon: chromosome
• Accession: NC_018068
• Organism: Desulfosporosinus acidiphilus SJ4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I4D9H8
• Locus tag: DESACI_RS17025
• Protein ID: WP_014828440.1
• Coordinates: 3721992..3722330 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DESACI_RS17020
• Protein ID: WP_014828439.1
• Coordinates: 3721765..3721998 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DESACI_RS17005	3717023..3719113	-	2091	WP_014828436.1	type I DNA topoisomerase	-
DESACI_RS17010	3719318..3720430	-	1113	WP_041276590.1	DNA-processing protein DprA	-
DESACI_RS17015	3720560..3721447	-	888	WP_014828438.1	Ku protein	-
DESACI_RS17020	3721765..3721998	+	234	WP_014828439.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DESACI_RS17025	3721992..3722330	+	339	WP_014828440.1	endoribonuclease MazF	Toxin
DESACI_RS17030	3722368..3722979	-	612	WP_014828441.1	DUF3786 domain-containing protein	-
DESACI_RS17035	3723277..3723465	+	189	WP_014828442.1	protein sspF	-
DESACI_RS17040	3723853..3725316	-	1464	WP_014828443.1	hypothetical protein	-
DESACI_RS17045	3725309..3726703	-	1395	WP_014828444.1	HAMP domain-containing histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• SpoVT-AbrB domain (6-47)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12698.89 Da
• Isoelectric Point: 7.8247

>T27935 WP_014828440.1 NC_018068:3721992-3722330 [Desulfosporosinus acidiphilus SJ4]
MVSQYVPERGDIVWIQLNPQLGHEQQGTRPALVLSAKEYNSKTGMGIFCPITSRVKGFPFEVKLPEKLEIRGVVLADQIK
SLDWRSRNASFICKVAEEIMEEVILKLEAIIK

Nucleotide

• Length: 339 bp

>T27935 NC_018068:3721992-3722330 [Desulfosporosinus acidiphilus SJ4]
ATGGTGAGCCAGTACGTTCCAGAACGCGGTGACATAGTATGGATTCAGTTAAATCCACAGCTCGGGCATGAGCAGCAAGG
AACTAGACCGGCTTTAGTTTTATCTGCAAAAGAATATAACAGCAAGACAGGGATGGGTATATTTTGCCCAATTACAAGCC
GGGTGAAGGGCTTCCCATTTGAAGTTAAACTTCCGGAGAAATTGGAAATCAGAGGTGTTGTCTTGGCCGACCAAATTAAA
TCCCTGGATTGGCGAAGCAGAAATGCCAGTTTCATATGCAAAGTGGCAGAGGAAATCATGGAAGAGGTTATTCTTAAACT
AGAAGCAATTATAAAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8770.00 Da
• Isoelectric Point: 4.1061

>AT27935 WP_014828439.1 NC_018068:3721765-3721998 [Desulfosporosinus acidiphilus SJ4]
MEAMARKWGNSIGIRIPASMANSIKITDGTPIDVELDGERIIITRKKFDLKELLAQIPDEYEPEEIDWGEPVGEEIW

Nucleotide

• Length: 234 bp

>AT27935 NC_018068:3721765-3721998 [Desulfosporosinus acidiphilus SJ4]
ATGGAAGCAATGGCACGAAAATGGGGAAATAGCATTGGCATAAGAATTCCTGCTTCAATGGCTAATTCAATAAAAATCAC
TGACGGAACCCCCATTGACGTTGAACTTGATGGCGAAAGGATCATAATTACCCGAAAAAAATTCGACTTAAAAGAATTAC
TTGCCCAAATTCCAGATGAATACGAGCCGGAAGAGATCGATTGGGGAGAACCCGTGGGAGAAGAGATATGGTGA

Structures

Toxin

• AlphaFold DB: I4D9H8