Detailed information of TA system TA027931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 14364..14880
• Replicon: chromosome
• Accession: NC_018028
• Organism: Pseudomonas stutzeri CCUG 29243

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I4CMH5
• Locus tag: A458_RS21870
• Protein ID: WP_014818489.1
• Coordinates: 14593..14880 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I4CMH4
• Locus tag: A458_RS21865
• Protein ID: WP_003284884.1
• Coordinates: 14364..14603 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A458_RS21850	10147..10812	+	666	WP_014818486.1	hypothetical protein	-
A458_RS44185	10889..11674	+	786	WP_202947490.1	hypothetical protein	-
A458_RS21855	11894..13021	+	1128	Protein_10	hypothetical protein	-
A458_RS21860	13114..13908	+	795	WP_014818488.1	NYN domain-containing protein	-
A458_RS21865	14364..14603	+	240	WP_003284884.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
A458_RS21870	14593..14880	+	288	WP_014818489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A458_RS21875	14925..17312	+	2388	WP_014818490.1	DEAD/DEAH box helicase family protein	-
A458_RS21880	17862..19424	+	1563	WP_014818491.1	alkyl hydroperoxide reductase subunit F	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11300.19 Da
• Isoelectric Point: 10.2579

>T27931 WP_014818489.1 NC_018028:14593-14880 [Pseudomonas stutzeri CCUG 29243]
MIYSLEFDARALKEWQKLGDTVRQQLKKKLAEVLLNPRIEANRLHSLPDCYKIKLRSSGYRLVYQVLDHEVVVFVVAVDR
REREQAYRKAAERLK

Nucleotide

• Length: 288 bp

>T27931 NC_018028:14593-14880 [Pseudomonas stutzeri CCUG 29243]
ATGATCTATAGCCTTGAGTTTGATGCGCGGGCACTGAAGGAATGGCAAAAACTGGGTGATACCGTTCGCCAGCAGCTGAA
GAAAAAGCTCGCTGAGGTGTTGCTGAATCCGCGCATTGAAGCCAATCGGCTCCATTCGCTGCCGGACTGCTACAAGATCA
AGCTGCGCAGCAGTGGTTATCGCCTGGTGTACCAGGTGCTTGACCACGAGGTGGTGGTGTTTGTGGTCGCGGTTGATCGC
CGTGAGCGTGAGCAGGCCTATCGCAAGGCAGCCGAGCGTCTTAAATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8994.40 Da
• Isoelectric Point: 4.6151

>AT27931 WP_003284884.1 NC_018028:14364-14603 [Pseudomonas stutzeri CCUG 29243]
MASINIRIDDELKARAYQELEKLGVTPSELMRQALQYVAERGQLPFRPVLMTEEDEALIATVRERLAAPQRVKVALDDL

Nucleotide

• Length: 240 bp

>AT27931 NC_018028:14364-14603 [Pseudomonas stutzeri CCUG 29243]
ATGGCGTCCATCAATATCCGCATCGACGACGAGCTTAAGGCGCGTGCTTATCAGGAGCTGGAAAAGCTTGGCGTCACCCC
CTCTGAGCTGATGCGCCAGGCCTTGCAGTATGTAGCCGAGCGCGGACAGCTGCCATTTCGCCCGGTGCTGATGACCGAGG
AAGATGAAGCGTTAATCGCTACGGTTCGTGAACGCCTGGCCGCTCCGCAGCGCGTGAAGGTTGCGTTGGATGATCTATAG

Structures

Toxin

• AlphaFold DB: I4CMH5

Antitoxin

• AlphaFold DB: I4CMH4