Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516647..517283 Replicon chromosome
Accession NZ_CP123977
Organism Bacillus subtilis subsp. subtilis strain WH60A

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QEP20_RS02635 Protein ID WP_003156187.1
Coordinates 516933..517283 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QEP20_RS02630 Protein ID WP_003225183.1
Coordinates 516647..516928 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEP20_RS02610 (QEP20_02610) 513006..513605 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
QEP20_RS02615 (QEP20_02615) 513700..514065 + 366 WP_015252768.1 holo-ACP synthase -
QEP20_RS02620 (QEP20_02620) 514231..515247 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
QEP20_RS02625 (QEP20_02625) 515362..516531 + 1170 WP_003234284.1 alanine racemase -
QEP20_RS02630 (QEP20_02630) 516647..516928 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QEP20_RS02635 (QEP20_02635) 516933..517283 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QEP20_RS02640 (QEP20_02640) 517398..518222 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
QEP20_RS02645 (QEP20_02645) 518227..518592 + 366 WP_015715277.1 RsbT antagonist protein RsbS -
QEP20_RS02650 (QEP20_02650) 518596..518997 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
QEP20_RS02655 (QEP20_02655) 519009..520016 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
QEP20_RS02660 (QEP20_02660) 520078..520407 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
QEP20_RS02665 (QEP20_02665) 520404..520886 + 483 WP_003234299.1 anti-sigma B factor RsbW -
QEP20_RS02670 (QEP20_02670) 520852..521640 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
QEP20_RS02675 (QEP20_02675) 521640..522239 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T279296 WP_003156187.1 NZ_CP123977:516933-517283 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT279296 WP_003225183.1 NZ_CP123977:516647-516928 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References