Detailed information of TA system TA027925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 428321..428949
• Replicon: plasmid pMYCCH.01
• Accession: NC_018022
• Organism: Mycobacterium chubuense NBB4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I4BSY2
• Locus tag: MYCCH_RS28085
• Protein ID: WP_014805661.1
• Coordinates: 428321..428731 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MYCCH_RS28090
• Protein ID: WP_041783913.1
• Coordinates: 428722..428949 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCCH_RS30500	424104..424326	-	223	Protein_418	hypothetical protein	-
MYCCH_RS28060	424414..424755	+	342	WP_014805659.1	MbcA/ParS/Xre antitoxin family protein	-
MYCCH_RS28065	424752..425312	+	561	WP_014805660.1	RES domain-containing protein	-
MYCCH_RS28070	425448..426853	-	1406	Protein_421	IS1380 family transposase	-
MYCCH_RS28080	427361..428075	+	715	Protein_422	AraC family transcriptional regulator	-
MYCCH_RS28085	428321..428731	-	411	WP_014805661.1	PIN domain nuclease	Toxin
MYCCH_RS28090	428722..428949	-	228	WP_041783913.1	hypothetical protein	Antitoxin
MYCCH_RS28095	429387..430202	-	816	WP_014805663.1	IS21-like element helper ATPase IstB	-
MYCCH_RS28100	430202..431416	-	1215	WP_014805664.1	IS21 family transposase	-
MYCCH_RS28105	433188..433709	-	522	WP_085980918.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fbpA	1..615278	615278
	-	inside	IScluster/Tn	-	-	425448..431416	5968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (11-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14944.21 Da
• Isoelectric Point: 4.8838

>T27925 WP_014805661.1 NC_018022:c428731-428321 [Mycolicibacterium chubuense NBB4]
MAVTNWIIDKSAYTRLSASRDATTWIERIERGLVRISTVTRLEIGYSFRTGQQARDETASPPLILMPVEYLTPVAEDRAV
AVQLLLADRGQHRAPAIPDLLVAATAELAGLTVLAMDKDFDLIAQITGQPVESLRV

Nucleotide

• Length: 411 bp

>T27925 NC_018022:c428731-428321 [Mycolicibacterium chubuense NBB4]
ATGGCGGTGACCAACTGGATCATCGACAAATCCGCCTACACACGACTGTCGGCGTCGCGCGACGCCACGACCTGGATCGA
ACGCATCGAGCGCGGCCTAGTTCGCATCAGCACCGTGACCCGGCTAGAGATCGGGTACTCGTTTCGTACCGGCCAGCAGG
CGCGTGACGAAACGGCGTCGCCTCCCCTAATCCTGATGCCGGTCGAATATTTGACACCAGTCGCTGAGGACCGCGCCGTG
GCGGTGCAGCTGCTCCTGGCCGATCGTGGCCAGCACCGCGCCCCCGCCATACCCGATCTCCTGGTAGCCGCCACCGCGGA
ATTAGCGGGATTGACCGTGCTTGCAATGGACAAGGATTTCGATCTCATCGCCCAGATCACCGGTCAGCCCGTCGAAAGCC
TGAGGGTGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8322.24 Da
• Isoelectric Point: 4.6028

>AT27925 WP_041783913.1 NC_018022:c428949-428722 [Mycolicibacterium chubuense NBB4]
ISDVLIRGLSEDAVARIDADAAARGLSRQEYLRQRFEREGTVSPVHRTLTLEDLRRAEAAAADLDDPGVMDSAWR

Nucleotide

• Length: 228 bp

>AT27925 NC_018022:c428949-428722 [Mycolicibacterium chubuense NBB4]
ATTAGCGACGTGCTGATTCGAGGACTGTCCGAGGATGCGGTGGCGCGTATCGACGCAGACGCGGCCGCCCGCGGCCTGTC
CCGCCAGGAATACCTGCGCCAACGGTTCGAGCGCGAAGGGACGGTGAGCCCGGTCCATCGCACACTGACGCTCGAGGATC
TGCGCCGCGCTGAGGCCGCCGCAGCCGACCTCGACGATCCGGGCGTGATGGACTCCGCATGGCGGTGA

Structures

Toxin

• AlphaFold DB: I4BSY2