Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 124895..126518 Replicon plasmid pTL012-1
Accession NZ_CP123952
Organism Escherichia coli strain TL012

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag QGM68_RS22595 Protein ID WP_000088046.1
Coordinates 125193..126518 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag QGM68_RS22590 Protein ID WP_024136327.1
Coordinates 124895..125188 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QGM68_RS22560 (QGM68_22560) 120767..122008 + 1242 WP_001053338.1 VWA domain-containing protein -
QGM68_RS22565 (QGM68_22565) 122099..122230 + 132 WP_000374059.1 hypothetical protein -
QGM68_RS22570 (QGM68_22570) 122571..122852 - 282 WP_000077926.1 hypothetical protein -
QGM68_RS22575 (QGM68_22575) 122902..123093 - 192 WP_000398480.1 hypothetical protein -
QGM68_RS22580 (QGM68_22580) 123185..123526 - 342 WP_001151575.1 hypothetical protein -
QGM68_RS22585 (QGM68_22585) 123899..124291 + 393 WP_000341066.1 hypothetical protein -
QGM68_RS22590 (QGM68_22590) 124895..125188 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
QGM68_RS22595 (QGM68_22595) 125193..126518 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
QGM68_RS22600 (QGM68_22600) 126579..126785 + 207 WP_000134171.1 hypothetical protein -
QGM68_RS22605 (QGM68_22605) 126887..127297 + 411 WP_000985911.1 hypothetical protein -
QGM68_RS22610 (QGM68_22610) 127310..128125 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
QGM68_RS22615 (QGM68_22615) 128379..128804 + 426 WP_001043843.1 hypothetical protein -
QGM68_RS22620 (QGM68_22620) 129353..129661 + 309 WP_001224687.1 hypothetical protein -
QGM68_RS22625 (QGM68_22625) 129677..130534 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
QGM68_RS22630 (QGM68_22630) 130596..130799 + 204 WP_001194555.1 hypothetical protein -
QGM68_RS22635 (QGM68_22635) 131141..131497 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaCTX-M-55 / blaTEM-1B / blaLAP-2 / aac(3)-IId / ant(3'')-Ia / lnu(F) / tet(A) / aph(6)-Id / floR / aph(3')-Ia / ARR-3 / dfrA14 - 1..260368 260368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T279244 WP_000088046.1 NZ_CP123952:125193-126518 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT279244 WP_024136327.1 NZ_CP123952:124895-125188 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References