Detailed information of TA system TA027924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-Xre
• Location: 424414..425312
• Replicon: plasmid pMYCCH.01
• Accession: NC_018022
• Organism: Mycobacterium chubuense NBB4

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: I4BSY1
• Locus tag: MYCCH_RS28065
• Protein ID: WP_014805660.1
• Coordinates: 424752..425312 (+)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: I4BSY0
• Locus tag: MYCCH_RS28060
• Protein ID: WP_014805659.1
• Coordinates: 424414..424755 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCCH_RS28050	421492..422199	-	708	WP_014805656.1	hypothetical protein	-
MYCCH_RS31245	422604..422762	+	159	WP_014805657.1	hypothetical protein	-
MYCCH_RS28055	423175..423978	-	804	WP_081495192.1	hypothetical protein	-
MYCCH_RS30500	424104..424326	-	223	Protein_418	hypothetical protein	-
MYCCH_RS28060	424414..424755	+	342	WP_014805659.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
MYCCH_RS28065	424752..425312	+	561	WP_014805660.1	RES domain-containing protein	Toxin
MYCCH_RS28070	425448..426853	-	1406	Protein_421	IS1380 family transposase	-
MYCCH_RS28080	427361..428075	+	715	Protein_422	AraC family transcriptional regulator	-
MYCCH_RS28085	428321..428731	-	411	WP_014805661.1	PIN domain nuclease	-
MYCCH_RS28090	428722..428949	-	228	WP_041783913.1	hypothetical protein	-
MYCCH_RS28095	429387..430202	-	816	WP_014805663.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fbpA	1..615278	615278
	-	inside	IScluster/Tn	-	-	425448..431416	5968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (29-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20386.66 Da
• Isoelectric Point: 4.6171

>T27924 WP_014805660.1 NC_018022:424752-425312 [Mycolicibacterium chubuense NBB4]
VSDGLDEDLVQRIDARGTAQWSGECFRYTGAHREPLSGEGARRFGGRWNPPLLFSAIYLADSAQACMVEVERAAQAASTT
PEQMLQARYRLHTIQATDMAVLDLTTAEARDAVGLEDDDVYGDDWSACQAVGHAAWFLHVQGVLVPAAGGVGLVITAYEQ
RTRPGQLQISHSEDLTPTLYRQRRDP

Nucleotide

• Length: 561 bp

>T27924 NC_018022:424752-425312 [Mycolicibacterium chubuense NBB4]
GTGAGCGATGGGCTCGACGAAGACCTGGTGCAGCGCATCGACGCGCGTGGCACCGCCCAGTGGTCGGGAGAGTGCTTCCG
ATACACCGGCGCCCATCGTGAGCCGTTATCAGGGGAGGGGGCGCGTAGATTCGGCGGCAGGTGGAACCCGCCCCTATTAT
TCTCGGCCATCTATCTGGCCGATTCCGCGCAAGCGTGCATGGTTGAAGTGGAACGCGCCGCCCAGGCCGCATCGACGACA
CCGGAGCAGATGCTTCAAGCCCGTTACCGGCTGCACACCATTCAAGCGACCGATATGGCGGTTCTGGATCTGACCACCGC
CGAAGCGCGTGACGCGGTGGGGCTCGAAGACGACGACGTCTACGGTGACGATTGGTCGGCCTGCCAAGCGGTGGGCCATG
CCGCGTGGTTTCTGCACGTGCAAGGAGTTCTGGTGCCGGCGGCCGGTGGAGTCGGCCTGGTCATCACCGCCTACGAACAG
CGCACCCGCCCTGGCCAACTCCAAATCAGTCACAGCGAAGACCTGACACCAACCCTGTACCGACAACGACGTGACCCCTA
A

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12237.00 Da
• Isoelectric Point: 5.0774

>AT27924 WP_014805659.1 NC_018022:424414-424755 [Mycolicibacterium chubuense NBB4]
MGANALASTVSSAIERLGLTYEEVGGIVDASARSVARWTSGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLAHGKPADLVRDGDYQRVLALIDAMAEGIFV

Nucleotide

• Length: 342 bp

>AT27924 NC_018022:424414-424755 [Mycolicibacterium chubuense NBB4]
ATGGGAGCTAATGCGCTCGCCTCGACCGTGTCGAGTGCGATCGAGCGCTTGGGATTGACCTACGAAGAGGTTGGGGGCAT
CGTCGATGCCTCGGCGCGGTCAGTGGCGCGCTGGACTTCAGGTCAGGTCGTCCCGCAACGCCTCAACAAGCAACGACTCA
TTGAACTGGCCTACGTCGCCGACGCCTTGGCCGAGGTCCTTCCCCGCGATCAGGCCAACGTCTGGATGTTCTCGCCAAAC
CGATTACTCGCGCACGGCAAGCCCGCTGACCTGGTGCGCGACGGCGATTACCAGCGCGTCTTGGCTCTCATCGACGCCAT
GGCCGAAGGGATCTTCGTGTGA

Structures

Toxin

• AlphaFold DB: I4BSY1

Antitoxin

• AlphaFold DB: I4BSY0