Detailed information of TA system TA027923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 39029..39678
• Replicon: plasmid pMYCCH.01
• Accession: NC_018022
• Organism: Mycobacterium chubuense NBB4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I4BRZ3
• Locus tag: MYCCH_RS26310
• Protein ID: WP_014805344.1
• Coordinates: 39029..39460 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I4BRZ4
• Locus tag: MYCCH_RS26315
• Protein ID: WP_014805345.1
• Coordinates: 39457..39678 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCCH_RS26295	35221..36675	-	1455	WP_014805341.1	ParB/RepB/Spo0J family partition protein	-
MYCCH_RS26300	37529..38113	-	585	WP_081495183.1	thermonuclease family protein	-
MYCCH_RS26305	38110..38349	-	240	WP_014805343.1	hypothetical protein	-
MYCCH_RS31125	38423..38740	-	318	WP_158021529.1	hypothetical protein	-
MYCCH_RS26310	39029..39460	-	432	WP_014805344.1	PIN domain nuclease	Toxin
MYCCH_RS26315	39457..39678	-	222	WP_014805345.1	hypothetical protein	Antitoxin
MYCCH_RS26320	39769..40050	+	282	WP_041783859.1	hypothetical protein	-
MYCCH_RS26325	40073..40561	-	489	WP_158021530.1	DNA-binding protein	-
MYCCH_RS29740	40554..41789	-	1236	WP_014805347.1	helix-turn-helix transcriptional regulator	-
MYCCH_RS26335	41923..42855	-	933	WP_158021531.1	tyrosine-type recombinase/integrase	-
MYCCH_RS26340	42975..44294	-	1320	WP_014805349.1	IS256 family transposase	-
MYCCH_RS30295	44307..44612	-	306	Protein_48	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fbpA	1..615278	615278
	-	flank	IS/Tn	-	-	42975..44294	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15738.78 Da
• Isoelectric Point: 5.1978

>T27923 WP_014805344.1 NC_018022:c39460-39029 [Mycolicibacterium chubuense NBB4]
VTETTWLIDKSALARLAQSVESETWSNRIERGLVHISNVTRLEVGYSAQSGDIARREFRESPLAAMPVEYLTPRIEDRAL
QVQLLLADRGQHRGPSIPDLIIAATAELSGLTILHVDKDFDTITAITGQPTTRLTYTSPSTPA

Nucleotide

• Length: 432 bp

>T27923 NC_018022:c39460-39029 [Mycolicibacterium chubuense NBB4]
GTGACAGAAACGACGTGGCTGATCGACAAATCAGCTCTGGCGCGCCTGGCTCAGAGCGTCGAATCAGAGACCTGGAGCAA
CCGAATCGAACGCGGCCTGGTCCATATCAGCAATGTGACACGCCTGGAAGTAGGGTATTCGGCTCAATCCGGCGATATCG
CTCGGCGTGAGTTTCGTGAATCTCCCTTAGCCGCAATGCCAGTCGAGTATCTGACACCACGAATTGAAGATCGTGCGCTG
CAGGTGCAGCTATTGCTAGCCGATCGCGGCCAGCATCGCGGGCCGTCGATCCCCGACCTCATCATCGCGGCCACTGCCGA
GCTTTCGGGATTGACCATCCTGCACGTCGACAAGGATTTCGACACCATCACCGCGATTACCGGACAACCGACAACTCGCC
TTACCTACACCAGCCCAAGCACTCCGGCATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8186.30 Da
• Isoelectric Point: 5.0162

>AT27923 WP_014805345.1 NC_018022:c39678-39457 [Mycolicibacterium chubuense NBB4]
MSDVLIRDIPDDVLAGLDARATELGLSRVEYIRRRLAQDARAARVPVTHDDLQRMGRAVAGLADDDLMRQAWQ

Nucleotide

• Length: 222 bp

>AT27923 NC_018022:c39678-39457 [Mycolicibacterium chubuense NBB4]
ATGAGTGATGTGTTGATCCGCGACATTCCTGATGACGTACTCGCTGGGCTCGATGCCCGAGCTACTGAGTTGGGTTTGTC
GCGGGTTGAGTACATTCGTCGCCGCCTGGCCCAGGATGCTCGCGCGGCGCGCGTCCCCGTCACGCACGACGATCTACAGC
GGATGGGGCGCGCCGTCGCGGGCCTGGCCGACGACGACCTGATGCGCCAGGCGTGGCAGTGA

Structures

Toxin

• AlphaFold DB: I4BRZ3

Antitoxin

• AlphaFold DB: I4BRZ4