Detailed information of TA system TA027921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 13356..13900
• Replicon: plasmid pMYCCH.01
• Accession: NC_018022
• Organism: Mycobacterium chubuense NBB4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I4BRW9
• Locus tag: MYCCH_RS26210
• Protein ID: WP_014805320.1
• Coordinates: 13622..13900 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I4BRW8
• Locus tag: MYCCH_RS26205
• Protein ID: WP_014805319.1
• Coordinates: 13356..13625 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCCH_RS26180	9768..10565	+	798	WP_014805316.1	IS21-like element helper ATPase IstB	-
MYCCH_RS26185	10624..10983	+	360	Protein_12	transposase	-
MYCCH_RS30265	11021..11604	-	584	Protein_13	IS30 family transposase	-
MYCCH_RS26190	11849..12136	+	288	Protein_14	transposase	-
MYCCH_RS26195	12190..12744	+	555	WP_014805317.1	hypothetical protein	-
MYCCH_RS26200	12916..13311	+	396	WP_158021525.1	relaxase domain-containing protein	-
MYCCH_RS26205	13356..13625	+	270	WP_014805319.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MYCCH_RS26210	13622..13900	+	279	WP_014805320.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MYCCH_RS30270	14242..14493	+	252	WP_014805321.1	type II toxin-antitoxin system ParD family antitoxin	-
MYCCH_RS26215	14490..14786	+	297	WP_041783793.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MYCCH_RS26220	14888..16438	-	1551	WP_162131316.1	hypothetical protein	-
MYCCH_RS26230	17486..17929	-	444	WP_014805325.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fbpA	1..615278	615278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10590.26 Da
• Isoelectric Point: 10.3000

>T27921 WP_014805320.1 NC_018022:13622-13900 [Mycolicibacterium chubuense NBB4]
VSEPQLWSVQLSPGALRALERLPHKVAAAIAEFITSALPTDPYIMSKPLRFELEGWRVARRGGYRVTFRALPDEHALYIG
WIEHRAHVYRRP

Nucleotide

• Length: 279 bp

>T27921 NC_018022:13622-13900 [Mycolicibacterium chubuense NBB4]
GTGAGCGAACCGCAGCTCTGGTCAGTTCAGCTGTCGCCGGGCGCCCTGCGGGCGCTGGAGCGGCTGCCACACAAGGTGGC
CGCCGCGATCGCCGAGTTCATCACCAGCGCGTTGCCGACCGACCCGTACATCATGTCCAAGCCGCTCCGGTTCGAGCTGG
AAGGGTGGCGCGTGGCACGCCGCGGCGGGTACCGCGTCACATTCCGAGCCCTCCCCGACGAACACGCCTTGTACATCGGC
TGGATCGAGCATCGGGCCCACGTCTATCGCCGCCCCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9605.73 Da
• Isoelectric Point: 4.6112

>AT27921 WP_014805319.1 NC_018022:13356-13625 [Mycolicibacterium chubuense NBB4]
MRTVPLSEAKDKLSALVEEADTTHEVIQITRHGRVAAVIMSADDLESLTETLHALTTPGVVDGLRQADADYAAGNTISGD
ELRRRYGLT

Nucleotide

• Length: 270 bp

>AT27921 NC_018022:13356-13625 [Mycolicibacterium chubuense NBB4]
ATGCGAACTGTACCGCTCAGTGAAGCCAAGGACAAGCTTTCGGCGCTCGTTGAGGAGGCCGACACCACCCATGAAGTCAT
TCAGATCACCCGGCATGGCCGCGTCGCGGCGGTCATCATGTCCGCCGACGATCTGGAGTCGCTTACCGAAACTCTGCACG
CGCTAACAACTCCCGGCGTGGTCGACGGGCTACGCCAGGCCGACGCCGACTACGCGGCCGGCAACACGATCTCCGGCGAC
GAGTTGCGCCGCCGCTACGGGCTCACGTGA

Structures

Toxin

• AlphaFold DB: I4BRW9

Antitoxin

• AlphaFold DB: I4BRW8