Detailed information of TA system TA027918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 3512564..3513129
• Replicon: chromosome
• Accession: NC_018020
• Organism: Turneriella parva DSM 21527

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: I4B9N0
• Locus tag: TURPA_RS16755
• Protein ID: WP_014803172.1
• Coordinates: 3512564..3512887 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: I4B9N1
• Locus tag: TURPA_RS16760
• Protein ID: WP_014804484.1
• Coordinates: 3512881..3513129 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TURPA_RS23570	3508687..3508872	-	186	WP_157210553.1	hypothetical protein	-
TURPA_RS16745	3510678..3511271	+	594	WP_157210554.1	hypothetical protein	-
TURPA_RS16750	3511675..3512304	+	630	WP_014804483.1	hypothetical protein	-
TURPA_RS16755	3512564..3512887	-	324	WP_014803172.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TURPA_RS16760	3512881..3513129	-	249	WP_014804484.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TURPA_RS16765	3513500..3513751	+	252	WP_014804485.1	DUF4258 domain-containing protein	-
TURPA_RS16770	3513763..3513981	+	219	WP_014804486.1	DUF2283 domain-containing protein	-
TURPA_RS16775	3514498..3514767	+	270	WP_014804487.1	hypothetical protein	-
TURPA_RS16780	3514790..3515140	+	351	WP_014804488.1	hypothetical protein	-
TURPA_RS16785	3515462..3516379	+	918	WP_014804489.1	restriction endonuclease	-
TURPA_RS16790	3516731..3516916	-	186	WP_157210555.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3455166..3670475	215309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-105)

Antitoxin

• SpoVT-AbrB domain (6-47)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12084.41 Da
• Isoelectric Point: 10.3758

>T27918 WP_014803172.1 NC_018020:c3512887-3512564 [Turneriella parva DSM 21527]
MVVEQYDVFLINLDPTIGHEIKKTRPCLIISPNEMNGNISTIIIAPMTTVSRNYPTRVKTVFKKKTGYIVLDQIRTVDKA
RLVKKLGKVNIEAIGSVKSVLREMLID

Nucleotide

• Length: 324 bp

>T27918 NC_018020:c3512887-3512564 [Turneriella parva DSM 21527]
ATGGTAGTGGAACAATACGACGTATTTCTCATAAATCTTGATCCGACTATCGGCCATGAGATTAAGAAAACCAGGCCATG
CCTCATCATATCACCAAATGAGATGAACGGTAATATTTCCACGATAATCATCGCCCCTATGACAACCGTTTCGCGAAATT
ATCCGACACGAGTGAAAACAGTATTCAAAAAGAAGACCGGATATATTGTCCTCGACCAGATTCGTACGGTCGATAAAGCG
CGTTTAGTTAAGAAGCTTGGTAAGGTCAATATTGAAGCCATTGGCTCAGTTAAGTCAGTGCTGAGAGAAATGCTTATAGA
TTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9485.99 Da
• Isoelectric Point: 9.6894

>AT27918 WP_014804484.1 NC_018020:c3513129-3512881 [Turneriella parva DSM 21527]
MILQIVKVGNSKGLRIPKSILEQYHIEEEVDVTSTKDGLLLKPIKSKARAGWAKKFKEMATNRDDRLLMPDFTDAADRDW
QW

Nucleotide

• Length: 249 bp

>AT27918 NC_018020:c3513129-3512881 [Turneriella parva DSM 21527]
ATGATCTTGCAGATAGTAAAGGTGGGCAACTCGAAAGGGCTGCGGATCCCCAAAAGTATTTTGGAACAATACCATATCGA
AGAAGAGGTGGATGTTACCTCGACGAAAGATGGACTTCTTTTGAAACCTATAAAAAGCAAGGCTCGAGCAGGTTGGGCAA
AGAAATTCAAAGAGATGGCGACAAACCGGGATGATAGGTTATTAATGCCCGATTTCACAGATGCGGCAGATCGAGACTGG
CAATGGTAG

Structures

Toxin

• AlphaFold DB: I4B9N0

Antitoxin

• AlphaFold DB: I4B9N1