Detailed information of TA system TA279164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 211884..213366
• Replicon: plasmid pAtH25_79a
• Accession: NZ_CP123849
• Organism: Agrobacterium fabacearum strain H25/79

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: G6L82_RS23915
• Protein ID: WP_236736276.1
• Coordinates: 212509..213366 (+)
• Length: 286 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: A0A1V2AF37
• Locus tag: G6L82_RS23910
• Protein ID: WP_035201040.1
• Coordinates: 211884..212495 (+)
• Length: 204 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6L82_RS23880 (G6L82_023880)	207167..207901	-	735	WP_063976958.1	IS21-like element helper ATPase IstB	-
G6L82_RS23885 (G6L82_023885)	207834..209402	-	1569	Protein_213	IS21 family transposase	-
G6L82_RS23890 (G6L82_023890)	209505..209846	+	342	WP_173995473.1	hypothetical protein	-
G6L82_RS23895 (G6L82_023895)	209882..210606	+	725	Protein_215	SDR family oxidoreductase	-
G6L82_RS23900 (G6L82_023900)	210638..210853	+	216	WP_236742549.1	shikimate kinase	-
G6L82_RS23905 (G6L82_023905)	210913..211612	+	700	Protein_217	IS6 family transposase	-
G6L82_RS23910 (G6L82_023910)	211884..212495	+	612	WP_035201040.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
G6L82_RS23915 (G6L82_023915)	212509..213366	+	858	WP_236736276.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G6L82_RS23920 (G6L82_023920)	213403..214018	-	616	Protein_220	IS6 family transposase	-
G6L82_RS23925 (G6L82_023925)	214122..215018	-	897	WP_173995474.1	transglutaminase family protein	-
G6L82_RS23930 (G6L82_023930)	215491..216159	-	669	WP_065706112.1	GntR family transcriptional regulator	-
G6L82_RS23935 (G6L82_023935)	216243..216863	+	621	WP_173995475.1	C39 family peptidase	-
G6L82_RS23940 (G6L82_023940)	216909..217976	+	1068	WP_019565647.1	D-alanine--D-alanine ligase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..628949	628949
	-	inside	IScluster/Tn	-	-	207167..214018	6851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (31-256)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (8-89)

Sequences

Toxin

Protein

• Length: 286 a.a.
• Molecular weight: 31901.61 Da
• Isoelectric Point: 4.8870

>T279164 WP_236736276.1 NZ_CP123849:212509-213366 [Agrobacterium fabacearum]
MAKSVKDRLLNIARKESRAFDVLLVRFALERLLYRLSISAHRDRFVLKGGMLVTVWVADDNRVTRDADFLGHGDADPACL
IANFGEIMAIESDDGLVFDINALAATVIREEMEYGGVRLKTAAYLERTRIPITIDIGFGDAMADATQRLDYPALLDFPAP
QVRSYPPATVIAEKFQAMVALGVLNGRMKDYYDLWAIPRAIDIPADDLDAAIRATFERRRTPIPKERPPGLSVEMFGDEG
KQRQWRAYAASLELDAVSLESIIERVWELVGQSCARIVARNENEA

Nucleotide

• Length: 858 bp

Antitoxin

Protein

• Length: 204 a.a.
• Molecular weight: 22244.77 Da
• Isoelectric Point: 10.5327

>AT279164 WP_035201040.1 NZ_CP123849:211884-212495 [Agrobacterium fabacearum]
MPSSQKAKLRSLLAARSMMRAHELREAGISAQTIARAVEGGEIERISRGVYQKRDAEVEENQILAEAIMRAPKGVIALVS
ALAFHGLTDQMPRRVWMAIGTSDWAPVQSYPPLRIVRFTEPYLRQGIENQVIAGIDVPVYSIPKTLADLFRNPKLVDRSV
AVEGLRAALQQRKSTPSAIAQAAKAGGAWKVMQPYLEALTSNG

Nucleotide

• Length: 612 bp

Structures

Antitoxin

• AlphaFold DB: A0A1V2AF37