Detailed information of TA system TA027911

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii(toxin)
• Location: 1035444..1037073
• Replicon: chromosome
• Accession: NC_018015
• Organism: Thermococcus sp. CL1

Toxin (Protein)

• Gene name: PygT
• Uniprot ID: I3ZU89
• Locus tag: CL1_RS05535
• Protein ID: WP_014788908.1
• Coordinates: 1036057..1037073 (+)
• Length: 339 a.a.

Antitoxin (Protein)

• Gene name: PygA
• Uniprot ID: I3ZU88
• Locus tag: CL1_RS05530
• Protein ID: WP_014788907.1
• Coordinates: 1035444..1036067 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CL1_RS05500 (CL1_1063)	1032392..1032871	+	480	WP_014788901.1	toll/interleukin-1 receptor domain-containing protein	-
CL1_RS05505 (CL1_1064)	1032896..1033195	+	300	WP_014788902.1	hypothetical protein	-
CL1_RS05510 (CL1_1065)	1033167..1033559	+	393	WP_014788903.1	hypothetical protein	-
CL1_RS05515 (CL1_1066)	1033663..1034217	-	555	WP_014788904.1	hypothetical protein	-
CL1_RS05520 (CL1_1067)	1034231..1034770	-	540	WP_048151988.1	hypothetical protein	-
CL1_RS05525 (CL1_1068)	1034745..1035332	-	588	WP_014788906.1	hypothetical protein	-
CL1_RS05530 (CL1_1069)	1035444..1036067	+	624	WP_014788907.1	hypothetical protein	Antitoxin
CL1_RS05535 (CL1_1070)	1036057..1037073	+	1017	WP_014788908.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CL1_RS05540 (CL1_1071)	1037066..1040452	+	3387	WP_014788909.1	helicase-related protein	-
CL1_RS05545 (CL1_1072)	1040514..1040960	+	447	WP_014788910.1	DUF365 domain-containing protein	-
CL1_RS05550 (CL1_1073)	1040957..1041388	-	432	WP_014788911.1	EVE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1033663..1054966	21303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (45-270)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 339 a.a.
• Molecular weight: 40009.93 Da
• Isoelectric Point: 7.8395

>T27911 WP_014788908.1 NC_018015:1036057-1037073 [Thermococcus cleftensis]
MLDDSLLMEIRLNERRSFARFVSKKTGIKSVDLVEWDYIIHTILKELEKDPTFRENYVFKGGTCLVKCHLGYYRFSRDLD
FAYRHSDELRNMSRSKLKKFLNGETGRIAETLKCVAKDFGLEFQYKDHSDFNNHRYFSFRIGPGWFREIVLYSPLGEKIK
VEVNYAERLAFRPKTLKANTLLSWKGVKLTPREYEKYIEFLGNYYPLSLTAYSDREILVEKVRALLTRKEFKLRDLYDLY
KLNQRGLRVSKYKKEIVNKIHDYLNLSSPAKEHLESSLQSIDEGTFLENIEGEMDRDIGLIIEPFEREEFLNFVGELKEE
LKGIVDDLKHLVEVEKDG

Nucleotide

• Length: 1017 bp

>T27911 NC_018015:1036057-1037073 [Thermococcus cleftensis]
ATGTTGGATGACTCGCTTCTTATGGAGATTCGCCTGAACGAGAGGAGAAGTTTTGCCAGGTTTGTCTCGAAGAAAACAGG
GATAAAATCTGTTGATCTCGTGGAGTGGGATTACATAATCCACACTATCTTAAAGGAGCTTGAGAAAGATCCCACATTCA
GGGAGAACTACGTTTTCAAAGGCGGAACCTGTCTCGTAAAGTGCCATCTCGGCTACTATCGCTTCAGCAGGGATTTAGAC
TTTGCATATAGGCACAGTGATGAGCTCAGGAATATGAGTAGGAGCAAGCTGAAGAAGTTCTTGAACGGGGAAACCGGCAG
GATTGCTGAGACCCTGAAGTGCGTGGCCAAGGATTTTGGTCTTGAGTTTCAATACAAAGATCATAGCGACTTCAACAACC
ATCGTTACTTTAGCTTCCGAATCGGCCCAGGCTGGTTTAGGGAGATAGTTCTCTACTCACCCTTGGGAGAGAAGATAAAG
GTGGAGGTAAACTACGCTGAGAGGCTTGCGTTCAGGCCAAAGACCCTGAAGGCGAACACCCTTCTGTCCTGGAAGGGAGT
CAAACTCACCCCGAGGGAGTATGAAAAGTACATTGAATTCCTCGGTAACTATTACCCACTCTCCCTCACTGCGTACTCCG
ACAGGGAGATTTTGGTGGAGAAAGTCAGGGCGCTGTTAACCCGAAAAGAGTTCAAACTCAGGGATCTCTATGACCTTTAC
AAGCTCAATCAGCGTGGCCTTAGGGTATCCAAGTACAAGAAGGAGATAGTGAACAAAATACATGACTACCTTAACTTGAG
CTCTCCGGCAAAGGAACATCTAGAGAGCTCCTTACAGTCCATTGATGAAGGCACCTTCCTCGAAAACATTGAAGGTGAGA
TGGACAGGGACATCGGTCTGATCATCGAGCCCTTCGAAAGGGAAGAGTTTTTGAACTTCGTTGGAGAGTTAAAAGAGGAA
CTCAAGGGTATTGTTGATGATTTGAAGCACTTGGTAGAGGTGGAGAAAGATGGCTGA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 24195.04 Da
• Isoelectric Point: 9.9942

>AT27911 WP_014788907.1 NC_018015:1035444-1036067 [Thermococcus cleftensis]
MKPVIQLLLTEFGGKVITREELERLASYFNEDVDYLVNYLIQYGYVIRILRGLYYVKTPIEFSTGGSPPIYRLLSLGMGK
ITKNWYFGLFTALVLNGLTHEGYTTVFIINDRLARPRPIQVNGTPVRIIRSRRNFFDFGIVARDSLKFSDLEKTLLDFLY
FSNYGTIPKNLALRVWREYIDNANTEKLHRYSERYPRSIRKVVENVG

Nucleotide

• Length: 624 bp

>AT27911 NC_018015:1035444-1036067 [Thermococcus cleftensis]
ATGAAGCCCGTCATCCAGCTCCTACTCACGGAGTTCGGGGGAAAGGTAATAACCCGCGAAGAACTTGAAAGGCTCGCCTC
GTACTTCAACGAGGACGTTGATTATCTGGTGAACTATCTCATCCAGTACGGCTACGTTATTCGTATCTTGCGGGGCCTCT
ACTACGTGAAAACACCGATAGAGTTCTCAACCGGTGGCTCTCCTCCAATCTACAGGCTACTATCCCTCGGAATGGGTAAG
ATAACGAAGAATTGGTACTTCGGTCTCTTTACGGCCCTGGTCCTCAACGGCCTTACCCATGAAGGGTACACGACGGTCTT
CATAATAAACGACAGACTCGCAAGACCAAGGCCTATTCAGGTGAATGGAACCCCAGTTAGGATAATCAGGAGCAGGAGGA
ACTTCTTTGACTTTGGAATAGTTGCCAGGGACTCACTAAAGTTCTCTGACCTCGAGAAGACCCTCCTTGACTTCCTGTAC
TTCTCCAACTACGGCACCATACCCAAGAACCTCGCCCTAAGGGTTTGGAGAGAATACATAGATAATGCCAATACAGAAAA
ACTCCACCGGTATTCCGAAAGGTATCCCCGCTCAATAAGGAAGGTGGTTGAGAATGTTGGATGA

Structures

Toxin

• AlphaFold DB: I3ZU89

Antitoxin

• AlphaFold DB: I3ZU88