Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 24081..24724 Replicon plasmid unnamed2
Accession NZ_CP123600
Organism Enterobacter cloacae strain RMCH-M23-N

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag QFB83_RS24500 Protein ID WP_000754567.1
Coordinates 24081..24497 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag QFB83_RS24505 Protein ID WP_023293777.1
Coordinates 24494..24724 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QFB83_RS24470 (QFB83_24470) 19267..19848 + 582 WP_032676110.1 TetR/AcrR family transcriptional regulator -
QFB83_RS24475 (QFB83_24475) 19853..20191 + 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
QFB83_RS24480 (QFB83_24480) 20221..20550 - 330 WP_008502229.1 thioredoxin family protein -
QFB83_RS24485 (QFB83_24485) 20763..21869 + 1107 WP_006687059.1 alkene reductase -
QFB83_RS24490 (QFB83_24490) 21935..22636 + 702 WP_280597657.1 DsbA family oxidoreductase -
QFB83_RS24495 (QFB83_24495) 22762..23730 + 969 WP_084832609.1 IS5-like element IS903B family transposase -
QFB83_RS24500 (QFB83_24500) 24081..24497 - 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
QFB83_RS24505 (QFB83_24505) 24494..24724 - 231 WP_023293777.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QFB83_RS24510 (QFB83_24510) 24862..25416 + 555 WP_161646454.1 hypothetical protein -
QFB83_RS24515 (QFB83_24515) 25504..25575 - 72 Protein_29 hypothetical protein -
QFB83_RS24520 (QFB83_24520) 25705..26910 + 1206 WP_032445154.1 AAA family ATPase -
QFB83_RS24525 (QFB83_24525) 26910..27884 + 975 WP_280597659.1 ParB family protein -
QFB83_RS24530 (QFB83_24530) 27966..29224 - 1259 Protein_32 Y-family DNA polymerase -
QFB83_RS24535 (QFB83_24535) 29224..29655 - 432 WP_006796638.1 translesion error-prone DNA polymerase V autoproteolytic subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..84591 84591
- inside IScluster/Tn - - 7454..23730 16276


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T279080 WP_000754567.1 NZ_CP123600:c24497-24081 [Enterobacter cloacae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T279080 NZ_CP123600:c24497-24081 [Enterobacter cloacae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT279080 WP_023293777.1 NZ_CP123600:c24724-24494 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT279080 NZ_CP123600:c24724-24494 [Enterobacter cloacae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTAG
ACAGGGCCGATCCGGACTTTATGGCGCAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References