Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 9559..10295 Replicon plasmid unnamed2
Accession NZ_CP123600
Organism Enterobacter cloacae strain RMCH-M23-N

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag QFB83_RS24420 Protein ID WP_003026803.1
Coordinates 9813..10295 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag QFB83_RS24415 Protein ID WP_003026799.1
Coordinates 9559..9825 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QFB83_RS24390 (QFB83_24390) 4642..5652 + 1011 WP_000200070.1 RepB family plasmid replication initiator protein -
QFB83_RS24395 (QFB83_24395) 6350..7090 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
QFB83_RS24400 (QFB83_24400) 7454..8422 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
QFB83_RS24405 (QFB83_24405) 8667..8870 + 204 WP_004213596.1 HHA domain-containing protein -
QFB83_RS24410 (QFB83_24410) 8884..9114 + 231 WP_023307222.1 hypothetical protein -
QFB83_RS24415 (QFB83_24415) 9559..9825 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
QFB83_RS24420 (QFB83_24420) 9813..10295 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
QFB83_RS24425 (QFB83_24425) 10474..11877 + 1404 WP_004113090.1 ISNCY family transposase -
QFB83_RS24430 (QFB83_24430) 11908..12825 - 918 WP_158003452.1 S-4TM family putative pore-forming effector -
QFB83_RS24435 (QFB83_24435) 12829..13827 - 999 WP_001610306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..84591 84591
- inside IScluster/Tn - - 7454..23730 16276


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T279079 WP_003026803.1 NZ_CP123600:9813-10295 [Enterobacter cloacae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT279079 WP_003026799.1 NZ_CP123600:9559-9825 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References