Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 43734..45357 Replicon plasmid unnamed1
Accession NZ_CP123599
Organism Enterobacter cloacae strain RMCH-M23-N

Toxin (Protein)


Gene name hipA Uniprot ID A0A746VM43
Locus tag QFB83_RS23470 Protein ID WP_058688097.1
Coordinates 44032..45357 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag QFB83_RS23465 Protein ID WP_032636197.1
Coordinates 43734..44027 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QFB83_RS23445 (QFB83_23445) 39272..39769 + 498 WP_000340139.1 membrane protein -
QFB83_RS23450 (QFB83_23450) 39774..41162 + 1389 WP_058688094.1 hypothetical protein -
QFB83_RS23455 (QFB83_23455) 41878..42735 + 858 WP_058688095.1 hypothetical protein -
QFB83_RS23460 (QFB83_23460) 42936..43409 - 474 WP_160515224.1 hypothetical protein -
QFB83_RS23465 (QFB83_23465) 43734..44027 + 294 WP_032636197.1 helix-turn-helix transcriptional regulator Antitoxin
QFB83_RS23470 (QFB83_23470) 44032..45357 + 1326 WP_058688097.1 type II toxin-antitoxin system HipA family toxin Toxin
QFB83_RS23475 (QFB83_23475) 45418..45624 + 207 WP_000134171.1 hypothetical protein -
QFB83_RS23480 (QFB83_23480) 45726..46136 + 411 WP_000985911.1 hypothetical protein -
QFB83_RS23485 (QFB83_23485) 46149..46964 + 816 WP_058688099.1 HNH endonuclease signature motif containing protein -
QFB83_RS23490 (QFB83_23490) 47218..47643 + 426 WP_001043851.1 hypothetical protein -
QFB83_RS23495 (QFB83_23495) 48195..48503 + 309 WP_001224686.1 hypothetical protein -
QFB83_RS23500 (QFB83_23500) 48519..49376 + 858 WP_058688100.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..212845 212845


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(155-404)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-68)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50296.12 Da        Isoelectric Point: 6.0237

>T279078 WP_058688097.1 NZ_CP123599:44032-45357 [Enterobacter cloacae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHDDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEEMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10989.46 Da        Isoelectric Point: 10.5348

>AT279078 WP_032636197.1 NZ_CP123599:43734-44027 [Enterobacter cloacae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTTSSERK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A746VM43

Antitoxin

Source ID Structure

References