Detailed information of TA system TA027907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/PRK09812-MazE
• Location: 2736285..2736886
• Replicon: chromosome
• Accession: NC_018012
• Organism: Thiocystis violascens DSM 198

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I3YBR3
• Locus tag: THIVI_RS12085
• Protein ID: WP_014778875.1
• Coordinates: 2736530..2736886 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: I3YBR2
• Locus tag: THIVI_RS12080
• Protein ID: WP_014778874.1
• Coordinates: 2736285..2736530 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THIVI_RS12050	2731467..2732450	-	984	WP_014778870.1	IS110 family transposase	-
THIVI_RS12055	2733256..2733525	+	270	WP_014778871.1	DUF4160 domain-containing protein	-
THIVI_RS12060	2733536..2733775	+	240	WP_014778872.1	DUF2442 domain-containing protein	-
THIVI_RS12065	2734368..2734595	-	228	WP_052315021.1	hypothetical protein	-
THIVI_RS12070	2734592..2735797	-	1206	Protein_2446	IS3 family transposase	-
THIVI_RS12080	2736285..2736530	+	246	WP_014778874.1	growth regulator	Antitoxin
THIVI_RS12085	2736530..2736886	+	357	WP_014778875.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THIVI_RS12090	2736996..2738066	-	1071	WP_014778876.1	DUF2333 family protein	-
THIVI_RS12095	2738432..2739307	-	876	WP_014778877.1	TauD/TfdA family dioxygenase	-
THIVI_RS12100	2739409..2740752	-	1344	WP_041446972.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2734592..2735602	1010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12782.77 Da
• Isoelectric Point: 8.5617

>T27907 WP_014778875.1 NC_018012:2736530-2736886 [Thiocystis violascens DSM 198]
MAYLPNRGDIVHLDFDPSAGREMKGPHFGLVVSGKVFNQQGLAMMCPISQGTAAAARTHGTVVTLMGAGTAIQGAVHCHQ
LKSLDWRARQVRFKEVAPREIVDDVLARVEAILFDRSA

Nucleotide

• Length: 357 bp

>T27907 NC_018012:2736530-2736886 [Thiocystis violascens DSM 198]
ATGGCGTACCTGCCGAATCGTGGCGACATCGTGCATCTGGATTTCGACCCTTCCGCCGGCCGCGAAATGAAAGGCCCGCA
CTTCGGTCTTGTCGTGAGCGGCAAGGTCTTCAACCAGCAAGGGCTGGCGATGATGTGCCCGATTTCCCAAGGTACTGCGG
CGGCGGCTCGCACCCATGGCACCGTGGTGACGCTGATGGGAGCGGGAACGGCGATCCAGGGAGCCGTTCACTGTCATCAA
TTGAAGTCACTCGACTGGCGTGCGCGGCAGGTCCGCTTCAAGGAGGTTGCGCCGCGAGAGATCGTCGATGACGTCCTGGC
CAGAGTCGAAGCCATTCTGTTTGATCGATCCGCGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8831.17 Da
• Isoelectric Point: 4.4865

>AT27907 WP_014778874.1 NC_018012:2736285-2736530 [Thiocystis violascens DSM 198]
MLQTLRKAGGSLVMTVPKAFIDQNALGEGSQVELHLLGRTMTVEAPPRPRYKLADLIAEMPDGLPRVDGWDDMPSVGLEN
D

Nucleotide

• Length: 246 bp

>AT27907 NC_018012:2736285-2736530 [Thiocystis violascens DSM 198]
ATGCTTCAGACCCTACGCAAAGCCGGTGGCTCGCTCGTGATGACAGTGCCCAAGGCCTTTATTGACCAGAATGCACTGGG
CGAAGGCTCCCAGGTTGAGCTGCATCTTCTTGGCAGGACCATGACGGTCGAAGCGCCACCCCGTCCGCGTTACAAGCTGG
CCGACCTGATCGCCGAGATGCCGGACGGTTTGCCGCGTGTCGACGGTTGGGATGACATGCCCTCGGTCGGACTGGAGAAC
GACTGA

Structures

Toxin

• AlphaFold DB: I3YBR3

Antitoxin

• AlphaFold DB: I3YBR2