Detailed information of TA system TA027904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 775829..776409
• Replicon: chromosome
• Accession: NC_018012
• Organism: Thiocystis violascens DSM 198

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I3Y6Z9
• Locus tag: THIVI_RS03475
• Protein ID: WP_014777261.1
• Coordinates: 775829..776167 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I3Y700
• Locus tag: THIVI_RS03480
• Protein ID: WP_014777262.1
• Coordinates: 776167..776409 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THIVI_RS03455	771860..772720	-	861	WP_014777257.1	ModD protein	-
THIVI_RS03460	773006..773413	-	408	WP_014777258.1	hypothetical protein	-
THIVI_RS03465	773682..773969	+	288	WP_014777259.1	co-chaperone GroES	-
THIVI_RS03470	774031..775665	+	1635	WP_014777260.1	chaperonin GroEL	-
THIVI_RS03475	775829..776167	-	339	WP_014777261.1	endoribonuclease MazF	Toxin
THIVI_RS03480	776167..776409	-	243	WP_014777262.1	PbsX family transcriptional regulator	Antitoxin
THIVI_RS03485	777191..779077	+	1887	WP_014777263.1	TonB-dependent vitamin B12 receptor	-
THIVI_RS23330	779271..779432	+	162	WP_083845692.1	bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase	-
THIVI_RS03495	779429..780484	+	1056	WP_014777264.1	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
THIVI_RS03500	780487..781284	+	798	WP_014777265.1	adenosylcobinamide-GDP ribazoletransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-109)

Antitoxin

• Antitoxin PemI-like (5-79)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12228.08 Da
• Isoelectric Point: 9.4825

>T27904 WP_014777261.1 NC_018012:c776167-775829 [Thiocystis violascens DSM 198]
MTTPYVPDEGDIVWMNFTPYSGHEQAGRRPAVVLSPKAYNRRAGLLVCVPITNQIKGYPFEVALAGADASGAALSDQVKS
LDWQTRQAERKGRATLSEIVEIKVKIKSLLGL

Nucleotide

• Length: 339 bp

>T27904 NC_018012:c776167-775829 [Thiocystis violascens DSM 198]
ATGACGACGCCCTACGTGCCCGACGAGGGCGACATCGTTTGGATGAACTTCACGCCATACAGCGGGCACGAGCAGGCTGG
ACGACGACCAGCCGTCGTCTTGAGTCCCAAGGCGTATAACCGTCGCGCCGGATTGCTCGTGTGTGTCCCCATCACCAACC
AGATCAAGGGCTACCCATTCGAGGTCGCGCTGGCGGGTGCAGATGCCAGCGGCGCCGCACTGTCGGATCAAGTCAAAAGC
CTGGACTGGCAGACTCGGCAGGCTGAGCGCAAGGGCAGGGCGACGCTCTCCGAAATCGTCGAAATCAAGGTCAAAATCAA
GTCGCTATTGGGACTGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8801.03 Da
• Isoelectric Point: 4.2550

>AT27904 WP_014777262.1 NC_018012:c776409-776167 [Thiocystis violascens DSM 198]
MAVQIVRKWGNSPAVRLPAAVMEAAHLMLDQVVDVRAEQGRVIIEPVAPTYSLEALLAGITDENRPDEQDFGFAEGREQL

Nucleotide

• Length: 243 bp

>AT27904 NC_018012:c776409-776167 [Thiocystis violascens DSM 198]
ATGGCCGTTCAAATCGTCCGGAAATGGGGCAACAGTCCTGCAGTGCGGCTGCCTGCCGCCGTGATGGAGGCGGCGCATCT
GATGCTCGATCAAGTCGTCGACGTGCGCGCGGAACAGGGTCGAGTCATCATTGAGCCAGTCGCGCCCACGTATTCGCTGG
AGGCGCTTCTCGCGGGAATCACCGACGAAAATCGTCCTGACGAACAGGATTTTGGATTCGCCGAAGGCAGGGAACAGCTC
TGA

Structures

Toxin

• AlphaFold DB: I3Y6Z9

Antitoxin

• AlphaFold DB: I3Y700