Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5028336..5028961 Replicon chromosome
Accession NZ_CP123430
Organism Klebsiella pneumoniae strain SH22PE16

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QEZ34_RS24440 Protein ID WP_002882817.1
Coordinates 5028336..5028719 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QEZ34_RS24445 Protein ID WP_004150355.1
Coordinates 5028719..5028961 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEZ34_RS24425 (5025702) 5025702..5026604 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QEZ34_RS24430 (5026601) 5026601..5027236 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QEZ34_RS24435 (5027233) 5027233..5028162 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QEZ34_RS24440 (5028336) 5028336..5028719 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QEZ34_RS24445 (5028719) 5028719..5028961 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QEZ34_RS24450 (5029166) 5029166..5030083 + 918 WP_065803683.1 alpha/beta hydrolase -
QEZ34_RS24455 (5030097) 5030097..5031038 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QEZ34_RS24460 (5031083) 5031083..5031520 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QEZ34_RS24465 (5031517) 5031517..5032377 - 861 WP_004146232.1 virulence factor BrkB family protein -
QEZ34_RS24470 (5032371) 5032371..5032970 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T278984 WP_002882817.1 NZ_CP123430:c5028719-5028336 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT278984 WP_004150355.1 NZ_CP123430:c5028961-5028719 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References