Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4430635..4431445 Replicon chromosome
Accession NZ_CP123430
Organism Klebsiella pneumoniae strain SH22PE16

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag QEZ34_RS21620 Protein ID WP_004178461.1
Coordinates 4430635..4431168 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag QEZ34_RS21625 Protein ID WP_002887278.1
Coordinates 4431179..4431445 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEZ34_RS21615 (4429466) 4429466..4430587 + 1122 WP_004214138.1 cupin domain-containing protein -
QEZ34_RS21620 (4430635) 4430635..4431168 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
QEZ34_RS21625 (4431179) 4431179..4431445 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
QEZ34_RS21630 (4431548) 4431548..4432981 - 1434 WP_023283180.1 Cu(+)/Ag(+) sensor histidine kinase -
QEZ34_RS21635 (4432971) 4432971..4433654 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
QEZ34_RS21640 (4433826) 4433826..4435211 + 1386 WP_023283179.1 efflux transporter outer membrane subunit -
QEZ34_RS21645 (4435229) 4435229..4435573 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T278981 WP_004178461.1 NZ_CP123430:c4431168-4430635 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT278981 WP_002887278.1 NZ_CP123430:c4431445-4431179 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References