Detailed information of TA system TA027895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 232874..233544
• Replicon: chromosome
• Accession: NC_018001
• Organism: Desulfurococcus fermentans DSM 16532

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I3XQD9
• Locus tag: DESFE_RS01280
• Protein ID: WP_014767067.1
• Coordinates: 232874..233278 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I3XQE0
• Locus tag: DESFE_RS01285
• Protein ID: WP_014767068.1
• Coordinates: 233275..233544 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DESFE_RS01255 (Desfe_0247)	228265..228684	-	420	WP_014767062.1	hypothetical protein	-
DESFE_RS01260 (Desfe_0248)	228697..230172	-	1476	WP_014767063.1	hypothetical protein	-
DESFE_RS01265 (Desfe_0249)	230177..231163	-	987	WP_014767064.1	hypothetical protein	-
DESFE_RS01270 (Desfe_0250)	231166..232158	-	993	WP_157861050.1	SPFH domain-containing protein	-
DESFE_RS01275 (Desfe_0251)	232155..232664	-	510	WP_014767066.1	hypothetical protein	-
DESFE_RS01280 (Desfe_0252)	232874..233278	-	405	WP_014767067.1	type II toxin-antitoxin system VapC family toxin	Toxin
DESFE_RS01285 (Desfe_0253)	233275..233544	-	270	WP_014767068.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DESFE_RS01295 (Desfe_0254)	234088..234507	-	420	WP_048815827.1	hypothetical protein	-
DESFE_RS01300 (Desfe_0255)	234517..234888	-	372	WP_014767070.1	hypothetical protein	-
DESFE_RS01305 (Desfe_0256)	234899..235273	-	375	WP_233418040.1	hypothetical protein	-
DESFE_RS01310 (Desfe_0257)	235406..236284	+	879	WP_014767072.1	hypothetical protein	-
DESFE_RS01315 (Desfe_0258)	236319..237482	+	1164	WP_048815828.1	AAA family ATPase	-
DESFE_RS01320 (Desfe_0259)	238074..238259	+	186	WP_173361975.1	chromatin protein Cren7	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

• SpoVT-AbrB domain (6-46)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15762.36 Da
• Isoelectric Point: 5.7467

>T27895 WP_014767067.1 NC_018001:c233278-232874 [Desulfurococcus amylolyticus DSM 16532]
MKVFIDSPLLIYLNTMADHKARIIYENFYIDMLTRYKPYTDVLVLDELIYISKKKYGIPYNITIEFIESNVLPYISITSL
GEEEYKQAIKFLSNYNLKPSDSLHLGAMINNGINTIVSEDREFDKIPIVRRLWI

Nucleotide

• Length: 405 bp

>T27895 NC_018001:c233278-232874 [Desulfurococcus amylolyticus DSM 16532]
TTGAAGGTCTTCATAGATTCCCCGCTTCTCATATACCTTAACACTATGGCAGATCACAAAGCTAGGATTATTTACGAGAA
CTTCTACATAGATATGCTAACTAGGTATAAGCCGTACACCGATGTACTTGTACTCGACGAGCTAATCTATATTTCTAAAA
AGAAGTACGGCATCCCATACAACATAACAATAGAGTTTATAGAATCAAATGTACTCCCATACATATCCATTACGAGTCTA
GGCGAAGAAGAGTATAAGCAAGCAATAAAATTTCTGTCTAACTACAACCTCAAACCCTCAGACTCGCTCCACTTAGGAGC
AATGATTAACAATGGTATAAACACCATTGTCAGCGAGGATAGAGAATTCGATAAAATACCCATAGTGAGGAGACTGTGGA
TATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10314.00 Da
• Isoelectric Point: 5.9461

>AT27895 WP_014767068.1 NC_018001:c233544-233275 [Desulfurococcus amylolyticus DSM 16532]
MTLKLRVGRKGYIILPKAVREVFGINEGDELIVEIRDGIVLKPARRSVDVEKLRESLRKHVEKLMDLPSRREPKPGELAE
AYLEEEFED

Nucleotide

• Length: 270 bp

>AT27895 NC_018001:c233544-233275 [Desulfurococcus amylolyticus DSM 16532]
TTGACTCTTAAGTTGAGGGTTGGGAGGAAAGGCTACATAATTTTACCGAAGGCTGTTCGCGAGGTTTTCGGTATTAATGA
GGGTGACGAGCTCATAGTCGAGATTAGAGATGGTATTGTACTCAAGCCTGCTAGGAGAAGTGTAGATGTAGAGAAGCTTA
GAGAATCTCTAAGAAAGCATGTCGAAAAACTCATGGATCTCCCTTCGAGGAGAGAGCCTAAGCCCGGCGAACTCGCAGAG
GCATACCTCGAGGAGGAGTTTGAGGATTGA

Structures

Toxin

• AlphaFold DB: I3XQD9

Antitoxin

• AlphaFold DB: I3XQE0