Detailed information of TA system TA027894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1166963..1167590
• Accession: NC_017992
• Organism: Thermoanaerobacterium saccharolyticum JW SL YS485

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I3VUG0
• Locus tag: TSAC_RS05805
• Protein ID: WP_014758038.1
• Coordinates: 1167240..1167590 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I3VUF9
• Locus tag: TSAC_RS05800
• Protein ID: WP_013297076.1
• Coordinates: 1166963..1167238 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TSAC_RS05780 (Tsac_1137)	1162730..1163137	+	408	WP_014758034.1	DUF6514 family protein	-
TSAC_RS05785 (Tsac_1138)	1163172..1164698	+	1527	WP_014758035.1	NAD(P)H-hydrate dehydratase	-
TSAC_RS05790 (Tsac_1139)	1164712..1165695	+	984	WP_038070095.1	outer membrane lipoprotein carrier protein LolA	-
TSAC_RS05795 (Tsac_1140)	1165709..1166875	+	1167	WP_014758037.1	alanine racemase	-
TSAC_RS05800 (Tsac_1141)	1166963..1167238	+	276	WP_013297076.1	ribbon-helix-helix protein, CopG family	Antitoxin
TSAC_RS05805 (Tsac_1142)	1167240..1167590	+	351	WP_014758038.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TSAC_RS05810 (Tsac_1143)	1167707..1168156	+	450	WP_014758039.1	hypothetical protein	-
TSAC_RS05815 (Tsac_1144)	1168270..1170291	+	2022	WP_014758040.1	DUF5693 family protein	-
TSAC_RS05820 (Tsac_1145)	1170329..1171408	+	1080	WP_014758041.1	polysaccharide pyruvyl transferase CsaB	-
TSAC_RS05825 (Tsac_1146)	1171411..1172142	+	732	WP_038070092.1	WecB/TagA/CpsF family glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12931.00 Da
• Isoelectric Point: 6.4714

>T27894 WP_014758038.1 NC_017992:1167240-1167590 [Thermoanaerobacterium saccharolyticum JW/SL-YS485]
MIVKRGDILYADLSPVIGSEQGGVRPVLVIQNDIGNKYSPTVIVSAITSQINKAKLPTHVEINGTEYGLNKDSVILLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T27894 NC_017992:1167240-1167590 [Thermoanaerobacterium saccharolyticum JW/SL-YS485]
ATGATTGTAAAGCGCGGAGATATTTTATATGCTGATTTAAGCCCTGTCATAGGCTCAGAACAGGGTGGTGTTAGGCCCGT
ACTCGTGATACAGAACGATATCGGCAATAAGTACAGCCCTACGGTTATTGTGTCAGCAATAACATCTCAGATAAACAAAG
CTAAATTGCCGACACATGTTGAGATAAATGGGACAGAATATGGTCTTAATAAGGATTCAGTTATTCTATTAGAACAAATA
AGGACTATAGACAAAAAGCGTCTAAGGGAAAAGATAGGTCATTTTGACCAAGAGATGATGGAAAAAGTAAATGAGGCATT
GCAGATAAGCCTTGGGTTGATAGATTTTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10514.31 Da
• Isoelectric Point: 7.7813

>AT27894 WP_013297076.1 NC_017992:1166963-1167238 [Thermoanaerobacterium saccharolyticum JW/SL-YS485]
VGETKRILVSLPESLLEEVDVLAAMENRNRSEFIREAMKLYIKERKKMKIRESMKKGYLEMAAINAELAELGLTADNECF
NGYEKKLKKCD

Nucleotide

• Length: 276 bp

>AT27894 NC_017992:1166963-1167238 [Thermoanaerobacterium saccharolyticum JW/SL-YS485]
GTGGGCGAGACAAAAAGAATACTCGTCAGTTTGCCGGAAAGCTTACTGGAAGAAGTTGATGTACTTGCAGCTATGGAAAA
TAGAAACCGCAGTGAATTTATAAGAGAAGCCATGAAATTGTACATTAAAGAGAGAAAGAAGATGAAAATAAGGGAGAGCA
TGAAAAAAGGGTACCTTGAGATGGCGGCTATAAATGCAGAACTTGCGGAATTGGGCCTAACCGCTGACAACGAATGCTTT
AACGGATACGAAAAGAAATTGAAAAAGTGTGATTGA

Structures

Toxin

• AlphaFold DB: I3VUG0

Antitoxin

• AlphaFold DB: I3VUF9