Detailed information of TA system TA027893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 163579..164165
• Replicon: plasmid pTM2
• Accession: NC_017966
• Organism: Tistrella mobilis KA081020-065

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I3TTS5
• Locus tag: TMO_RS21565
• Protein ID: WP_014752916.1
• Coordinates: 163878..164165 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I3TTS4
• Locus tag: TMO_RS21560
• Protein ID: WP_014752915.1
• Coordinates: 163579..163881 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMO_RS21540	158781..160622	+	1842	WP_014752911.1	dihydroxy-acid dehydratase	-
TMO_RS21545	160875..161813	+	939	WP_041607322.1	ABC transporter substrate-binding protein	-
TMO_RS21550	161919..162695	+	777	WP_041607324.1	ABC transporter permease	-
TMO_RS21555	162692..163480	+	789	WP_014752914.1	ABC transporter ATP-binding protein	-
TMO_RS21560	163579..163881	+	303	WP_014752915.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
TMO_RS21565	163878..164165	+	288	WP_014752916.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TMO_RS21570	164190..165971	-	1782	WP_014752917.1	pyruvate oxidase	-
TMO_RS21575	166136..167419	+	1284	WP_014752918.1	cytochrome P450	-
TMO_RS21580	167454..167984	+	531	WP_014752919.1	superoxide dismutase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..690188	690188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-52)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10908.49 Da
• Isoelectric Point: 8.6989

>T27893 WP_014752916.1 NC_017966:163878-164165 [Tistrella mobilis KA081020-065]
VRIIWSRFAALDRERIFDRIAAHDLGAAIGIDERIQQQSSDLARFPQRGRPGRIPGTRELVIHGTPYILAYRPGEETVRL
LRLLHGAQLWPDELE

Nucleotide

• Length: 288 bp

>T27893 NC_017966:163878-164165 [Tistrella mobilis KA081020-065]
GTGAGGATCATCTGGTCCCGCTTCGCGGCCCTGGACCGCGAGCGGATTTTCGACCGCATCGCCGCGCATGATCTTGGCGC
GGCGATCGGTATCGATGAACGAATTCAGCAGCAGTCGTCGGATCTGGCCCGGTTCCCGCAGCGGGGCCGGCCGGGCAGGA
TCCCCGGGACCCGCGAACTGGTCATCCACGGCACGCCTTACATCCTGGCCTATCGGCCGGGTGAAGAGACCGTCCGGCTC
CTGCGCCTGCTGCACGGCGCCCAGCTCTGGCCCGACGAGCTGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10552.96 Da
• Isoelectric Point: 5.7048

>AT27893 WP_014752915.1 NC_017966:163579-163881 [Tistrella mobilis KA081020-065]
MAVTALVQARIDAEVKEKASAVLDGVGLTLSDVVRIVLTRVAREGALPPGLVTDPATHDAWFRARVQEALDDTAGDLSHD
EVNARLAARREAALRRAGGA

Nucleotide

• Length: 303 bp

>AT27893 NC_017966:163579-163881 [Tistrella mobilis KA081020-065]
ATGGCGGTCACGGCTCTGGTTCAGGCGCGCATCGACGCCGAGGTGAAGGAGAAGGCGAGTGCGGTGCTCGACGGTGTCGG
GCTCACCCTCTCGGATGTCGTGCGTATCGTCCTGACCCGGGTGGCGCGGGAAGGGGCACTGCCCCCCGGCCTCGTCACCG
ATCCGGCCACCCATGATGCCTGGTTCCGCGCCCGGGTGCAGGAGGCGCTCGACGACACGGCCGGCGATCTCTCTCATGAC
GAGGTGAACGCCCGTCTCGCCGCCCGCCGGGAGGCAGCGCTCCGGCGGGCCGGCGGCGCGTGA

Structures

Toxin

• AlphaFold DB: I3TTS5

Antitoxin

• AlphaFold DB: I3TTS4