Detailed information of TA system TA027888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 127653..128320
• Replicon: chromosome
• Accession: NC_017964
• Organism: Advenella kashmirensis WT001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: TKWG_RS00600
• Protein ID: WP_202947781.1
• Coordinates: 127934..128320 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I3U761
• Locus tag: TKWG_RS00595
• Protein ID: WP_014748940.1
• Coordinates: 127653..127922 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TKWG_RS00570	123352..124116	+	765	WP_014748938.1	ABC transporter ATP-binding protein	-
TKWG_RS00575	124119..124885	+	767	Protein_120	ABC transporter permease	-
TKWG_RS00580	125118..125638	-	521	Protein_121	MarR family transcriptional regulator	-
TKWG_RS00585	125772..126667	-	896	Protein_122	LysR family transcriptional regulator	-
TKWG_RS00590	126695..127444	-	750	WP_041709709.1	aspartate/glutamate racemase family protein	-
TKWG_RS00595	127653..127922	+	270	WP_014748940.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TKWG_RS00600	127934..128320	+	387	WP_202947781.1	type II toxin-antitoxin system VapC family toxin	Toxin
TKWG_RS00605	128435..128972	+	538	Protein_126	AAA family ATPase	-
TKWG_RS00610	128965..129561	+	597	WP_202947753.1	DUF1311 domain-containing protein	-
TKWG_RS00615	129676..130154	+	479	Protein_128	GNAT family N-acetyltransferase	-
TKWG_RS22115	130493..130651	+	159	WP_041708838.1	DUF1328 domain-containing protein	-
TKWG_RS00625	130717..130923	-	207	WP_014748945.1	CsbD family protein	-
TKWG_RS00630	131259..131441	+	183	WP_014748946.1	DUF3008 family protein	-
TKWG_RS00635	131481..131981	-	501	WP_041708841.1	nicotinamide-nucleotide amidohydrolase family protein	-
TKWG_RS00640	132426..132950	+	525	WP_014748949.1	cytochrome b	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-112)

Antitoxin

• SpoVT-AbrB domain (18-58)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13603.68 Da
• Isoelectric Point: 4.7953

>T27888 WP_202947781.1 NC_017964:127934-128320 [Advenella kashmirensis WT001]
MLDTNIVSELARNPQGKTAQQIAIAGAETICVSIITACELRYGCARKKSPRLSAQIEAILGSVQVLAFDVPADTEYGVIR
EQLEAAGKPIGPNDLLIAAHAYAIGAILVTGNVSEFGRIQGLQIENWL

Nucleotide

• Length: 387 bp

>T27888 NC_017964:127934-128320 [Advenella kashmirensis WT001]
ATGCTCGATACGAATATCGTATCTGAATTAGCCAGAAACCCTCAGGGCAAGACTGCCCAGCAGATTGCAATTGCTGGCGC
TGAAACCATTTGCGTCAGCATCATCACAGCCTGCGAATTACGATATGGTTGTGCGAGAAAAAAGTCGCCCCGGTTGTCAG
CACAGATCGAAGCAATTCTTGGGAGTGTACAAGTGCTTGCATTTGATGTGCCCGCCGATACTGAGTATGGCGTGATACGT
GAACAACTGGAAGCTGCGGGGAAACCTATCGGCCCAAACGATCTACTGATTGCAGCACACGCCTATGCTATTGGAGCCAT
ACTAGTGACGGGCAATGTCAGCGAGTTTGGTCGCATTCAAGGCTTGCAGATAGAAAACTGGCTATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10114.77 Da
• Isoelectric Point: 5.7117

>AT27888 WP_014748940.1 NC_017964:127653-127922 [Advenella kashmirensis WT001]
MTVLRKLDPALAREARLFRNNKSQAVRIPADFELPGKRVLIHREGDKLIIEPIQHKNLVEVLAALEPLAPEDQFPQVDDT
LLPLKDTDL

Nucleotide

• Length: 270 bp

>AT27888 NC_017964:127653-127922 [Advenella kashmirensis WT001]
ATGACAGTACTGCGAAAGTTGGACCCCGCATTGGCAAGAGAAGCCAGGCTTTTTCGCAATAATAAAAGCCAGGCGGTTCG
CATCCCCGCAGACTTTGAATTACCAGGTAAAAGGGTTTTAATTCACCGGGAAGGGGACAAATTAATCATTGAGCCGATTC
AACATAAAAACCTGGTCGAAGTGCTGGCAGCGCTCGAGCCGCTTGCTCCCGAGGATCAGTTTCCGCAAGTCGATGACACA
CTCCTGCCGCTCAAGGATACCGATCTGTGA

Structures

Antitoxin

• AlphaFold DB: I3U761