Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 241890..242497 Replicon plasmid 3
Accession NC_017963
Organism Enterococcus faecium DO

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag HMPREF0351_RS14845 Protein ID WP_002321032.1
Coordinates 241890..242240 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag HMPREF0351_RS14850 Protein ID WP_002287514.1
Coordinates 242234..242497 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMPREF0351_RS14815 237540..238730 + 1191 WP_002288791.1 IS256 family transposase -
HMPREF0351_RS14820 238785..238994 - 210 WP_044051725.1 hypothetical protein -
HMPREF0351_RS14825 239168..239785 - 618 Protein_232 recombinase family protein -
HMPREF0351_RS14830 240276..240698 + 423 WP_002288787.1 hypothetical protein -
HMPREF0351_RS14835 240708..240911 + 204 WP_002299573.1 hypothetical protein -
HMPREF0351_RS14840 241252..241866 + 615 WP_002298870.1 recombinase family protein -
HMPREF0351_RS14845 241890..242240 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HMPREF0351_RS14850 242234..242497 - 264 WP_002287514.1 hypothetical protein Antitoxin
HMPREF0351_RS14855 242838..243017 - 180 WP_002298867.1 hypothetical protein -
HMPREF0351_RS14860 243462..243899 - 438 WP_002287515.1 hypothetical protein -
HMPREF0351_RS14865 243915..244166 - 252 WP_002287517.1 hypothetical protein -
HMPREF0351_RS14870 244166..244372 - 207 WP_014748825.1 hypothetical protein -
HMPREF0351_RS14875 244815..245075 - 261 WP_014748826.1 hypothetical protein -
HMPREF0351_RS14880 245230..245352 + 123 WP_002295624.1 putative holin-like toxin -
HMPREF0351_RS14885 245511..245768 - 258 WP_002390115.1 hypothetical protein -
HMPREF0351_RS14890 245790..246275 - 486 WP_002287518.1 single-stranded DNA-binding protein -
HMPREF0351_RS14895 246278..247177 - 900 WP_002287519.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..251926 251926


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T27887 WP_002321032.1 NC_017963:c242240-241890 [Enterococcus faecium DO]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T27887 NC_017963:c242240-241890 [Enterococcus faecium DO]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
GCCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT27887 WP_002287514.1 NC_017963:c242497-242234 [Enterococcus faecium DO]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT27887 NC_017963:c242497-242234 [Enterococcus faecium DO]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References