Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 61687..62206 Replicon plasmid 2
Accession NC_017962
Organism Enterococcus faecium DO

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag HMPREF0351_RS13610 Protein ID WP_000588503.1
Coordinates 61687..61944 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag HMPREF0351_RS13615 Protein ID WP_000388479.1
Coordinates 61937..62206 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMPREF0351_RS13580 56725..57015 - 291 WP_000026576.1 hypothetical protein -
HMPREF0351_RS13585 57151..57330 - 180 WP_001021554.1 hypothetical protein -
HMPREF0351_RS13590 57327..57677 - 351 WP_000568378.1 hypothetical protein -
HMPREF0351_RS13595 57670..58995 - 1326 WP_000969590.1 Y-family DNA polymerase -
HMPREF0351_RS13600 59445..60059 + 615 WP_001261742.1 recombinase family protein -
HMPREF0351_RS13605 60224..61228 - 1005 WP_000455809.1 hypothetical protein -
HMPREF0351_RS13610 61687..61944 - 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
HMPREF0351_RS13615 61937..62206 - 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HMPREF0351_RS13620 62337..62516 - 180 WP_001120991.1 hypothetical protein -
HMPREF0351_RS13625 62897..63592 - 696 WP_002326819.1 hypothetical protein -
HMPREF0351_RS13630 63654..64334 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
HMPREF0351_RS13635 64389..64670 - 282 Protein_80 HTH domain-containing protein -
HMPREF0351_RS13645 64946..65740 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3')-III / ant(6)-Ia / erm(B) / cat(pC233) - 1..66247 66247
- flank IS/Tn aph(3')-III - 63648..65731 2083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T27885 WP_000588503.1 NC_017962:c61944-61687 [Enterococcus faecium DO]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T27885 NC_017962:c61944-61687 [Enterococcus faecium DO]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT27885 WP_000388479.1 NC_017962:c62206-61937 [Enterococcus faecium DO]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT27885 NC_017962:c62206-61937 [Enterococcus faecium DO]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References