Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 514735..515306 Replicon chromosome
Accession NC_017960
Organism Enterococcus faecium DO

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag HMPREF0351_RS02465 Protein ID WP_002286801.1
Coordinates 514965..515306 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag HMPREF0351_RS02460 Protein ID WP_002323011.1
Coordinates 514735..514965 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMPREF0351_RS02430 509868..511199 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
HMPREF0351_RS02435 511221..511847 + 627 WP_002286816.1 cysteine hydrolase -
HMPREF0351_RS02440 512030..512611 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
HMPREF0351_RS02445 513343..513690 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
HMPREF0351_RS02450 513666..513905 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
HMPREF0351_RS02455 514110..514448 - 339 WP_002286804.1 hypothetical protein -
HMPREF0351_RS02460 514735..514965 + 231 WP_002323011.1 hypothetical protein Antitoxin
HMPREF0351_RS02465 514965..515306 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HMPREF0351_RS02470 516156..516341 + 186 WP_002304207.1 hypothetical protein -
HMPREF0351_RS02475 516846..517040 + 195 WP_002297028.1 hypothetical protein -
HMPREF0351_RS02480 517231..517480 + 250 Protein_494 transposase -
HMPREF0351_RS02485 517724..518554 - 831 WP_002286789.1 manganese catalase family protein -
HMPREF0351_RS02490 518762..520096 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T27884 WP_002286801.1 NC_017960:514965-515306 [Enterococcus faecium DO]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T27884 NC_017960:514965-515306 [Enterococcus faecium DO]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT27884 WP_002323011.1 NC_017960:514735-514965 [Enterococcus faecium DO]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT27884 NC_017960:514735-514965 [Enterococcus faecium DO]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References