Detailed information of TA system TA027881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 347114..347766
• Replicon: plasmid pTM3
• Accession: NC_017958
• Organism: Tistrella mobilis KA081020-065

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: TMO_RS25375
• Protein ID: WP_041607598.1
• Coordinates: 347114..347521 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I3TVZ0
• Locus tag: TMO_RS25380
• Protein ID: WP_014747917.1
• Coordinates: 347518..347766 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMO_RS30005	343422..345497	+	2076	WP_014747914.1	iron-containing redox enzyme family protein	-
TMO_RS25370	345494..347092	+	1599	WP_014747915.1	hypothetical protein	-
TMO_RS25375	347114..347521	-	408	WP_041607598.1	type II toxin-antitoxin system VapC family toxin	Toxin
TMO_RS25380	347518..347766	-	249	WP_014747917.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TMO_RS25385	347876..349150	-	1275	WP_014747918.1	glycerate kinase	-
TMO_RS25390	349350..350093	+	744	WP_014747919.1	creatininase family protein	-
TMO_RS25395	350106..350867	+	762	WP_014747920.1	N-formylglutamate amidohydrolase	-
TMO_RS25400	350920..351222	+	303	WP_014747921.1	DUF1244 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1126962	1126962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-81)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14787.98 Da
• Isoelectric Point: 5.1599

>T27881 WP_041607598.1 NC_017958:c347521-347114 [Tistrella mobilis KA081020-065]
MIVDTSAMVAILYREAEAEAFVNFIHDADICRMSVANYLELSMVIDKQLGSTGMQQADLFIRRAGIVIEPVTVEQGHLAR
LAFLNFGKGRHKAGLNYGDCFAYALAKDMAEPLLFKGNDFGETDLPRAAPASSSR

Nucleotide

• Length: 408 bp

>T27881 NC_017958:c347521-347114 [Tistrella mobilis KA081020-065]
ATGATTGTGGATACCTCCGCAATGGTCGCGATTCTTTATCGAGAGGCGGAGGCCGAGGCCTTTGTGAATTTTATACATGA
TGCCGATATCTGTCGAATGAGCGTCGCCAATTATCTTGAGTTGTCGATGGTGATCGATAAGCAGCTTGGCTCGACGGGAA
TGCAGCAGGCTGATCTCTTCATTCGCCGGGCAGGCATTGTGATCGAGCCGGTGACTGTGGAGCAGGGGCATCTTGCTCGT
CTGGCATTTCTGAATTTCGGAAAAGGACGCCACAAGGCGGGTCTCAATTATGGTGATTGTTTTGCTTATGCCCTGGCGAA
GGACATGGCAGAGCCTTTGCTTTTTAAGGGCAATGACTTCGGCGAGACGGATCTTCCAAGAGCAGCGCCGGCATCGTCAT
CCCGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9123.39 Da
• Isoelectric Point: 8.5565

>AT27881 WP_014747917.1 NC_017958:c347766-347518 [Tistrella mobilis KA081020-065]
MSLNVKDPEARRLAQAIADATGQSMSRVVTEALRERYARLRQPSAKASVEELLAIADHAAAHVKRPYLEHGDLLYDERGL
PK

Nucleotide

• Length: 249 bp

>AT27881 NC_017958:c347766-347518 [Tistrella mobilis KA081020-065]
ATGAGCCTGAACGTCAAAGATCCGGAAGCCCGGCGTCTGGCGCAGGCTATTGCCGATGCGACCGGCCAAAGCATGAGCCG
CGTGGTGACGGAAGCCCTGCGCGAGCGCTACGCACGGCTCCGACAGCCCTCCGCCAAGGCGAGCGTCGAGGAGCTTCTTG
CCATTGCAGATCATGCTGCAGCCCACGTAAAGCGCCCATATCTTGAACATGGCGATTTGCTTTACGATGAACGTGGGCTG
CCGAAATGA

Structures

Antitoxin

• AlphaFold DB: I3TVZ0