Detailed information of TA system TA027880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 46099..47141
• Replicon: plasmid pTM3
• Accession: NC_017958
• Organism: Tistrella mobilis KA081020-065

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: TMO_RS24020
• Protein ID: WP_003109777.1
• Coordinates: 46566..47141 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: TMO_RS24015
• Protein ID: WP_003050245.1
• Coordinates: 46099..46569 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMO_RS23980	41491..42909	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
TMO_RS23985	42899..43810	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
TMO_RS23990	43807..44499	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
TMO_RS23995	44496..44894	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
TMO_RS24000	44906..45265	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
TMO_RS24005	45282..45515	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
TMO_RS24010	45512..45895	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
TMO_RS24015	46099..46569	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
TMO_RS24020	46566..47141	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
TMO_RS24025	47159..48073	+	915	WP_003105629.1	AAA family ATPase	-
TMO_RS24030	48070..48540	+	471	WP_003105626.1	hypothetical protein	-
TMO_RS24035	48537..49037	+	501	WP_003090159.1	hypothetical protein	-
TMO_RS24040	49037..49939	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
TMO_RS24045	49978..50703	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1126962	1126962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T27880 WP_003109777.1 NC_017958:46566-47141 [Tistrella mobilis KA081020-065]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T27880 NC_017958:46566-47141 [Tistrella mobilis KA081020-065]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT27880 WP_003050245.1 NC_017958:46099-46569 [Tistrella mobilis KA081020-065]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT27880 NC_017958:46099-46569 [Tistrella mobilis KA081020-065]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68