Detailed information of TA system TA027879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 671177..672684
• Replicon: plasmid pTM1
• Accession: NC_017957
• Organism: Tistrella mobilis KA081020-065

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: I3TTB3
• Locus tag: TMO_RS20790
• Protein ID: WP_014747678.1
• Coordinates: 671177..672445 (-)
• Length: 423 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: I3TTB4
• Locus tag: TMO_RS20795
• Protein ID: WP_014747679.1
• Coordinates: 672442..672684 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMO_RS20785	667452..671111	+	3660	WP_014747677.1	hypothetical protein	-
TMO_RS20790	671177..672445	-	1269	WP_014747678.1	type II toxin-antitoxin system HipA family toxin	Toxin
TMO_RS20795	672442..672684	-	243	WP_014747679.1	helix-turn-helix transcriptional regulator	Antitoxin
TMO_RS20800	672882..675002	-	2121	WP_014747680.1	catalase	-
TMO_RS20805	675164..675652	-	489	WP_014747681.1	CBS domain-containing protein	-
TMO_RS20810	675558..676556	+	999	WP_145978320.1	UdgX family uracil-DNA binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..692456	692456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (148-381)

Antitoxin

• Transcription regulator, HipB-like (10-64)

Sequences

Toxin

Protein

• Length: 423 a.a.
• Molecular weight: 45204.01 Da
• Isoelectric Point: 5.7913

>T27879 WP_014747678.1 NC_017957:c672445-671177 [Tistrella mobilis KA081020-065]
MTRALDVWWDGRLVGQLTQNPHGELGFAYAPEWLGDAEMPPLSASLPKRAEPFSRRECRPFFGGLLPEEGQRDAVAQALG
VSRANDFALLDRLGGDVAGALQLLPPGEPQMGLADERQASPLDDAALIRVLEALPLRPLLAGERGLRLSLAGAQSKVPVV
LVDGAVALPVPGQPTTHILKPPISRFAATTENEAFVMCLAAAIGLDVAPVEARIVQGRPFLLVQRYDRDIGGDGAVHRIH
QEDFCQALGVPPEIKYASEGGPTFKDCFALLRRVAARPAVDVLKLLDAVIFNVIAGNADAHGKNFSILYDTGGPRLAPLY
DLLATVAYPDLSPNFAMKIGKRATLAELDAKGWATFAAETGLGLPLIRRRVAEISKGVLEKATAVAEELSPSGLDRRASE
QFADMIRDRAERCALTVGGRAR

Nucleotide

• Length: 1269 bp

>T27879 NC_017957:c672445-671177 [Tistrella mobilis KA081020-065]
ATGACGCGCGCCCTCGATGTCTGGTGGGACGGGCGCCTGGTGGGCCAGCTGACACAGAACCCGCACGGTGAACTCGGCTT
CGCCTATGCGCCGGAGTGGCTGGGCGATGCAGAGATGCCGCCCTTGTCCGCCTCGCTGCCGAAGCGGGCGGAGCCGTTCA
GTCGTCGCGAATGTCGGCCGTTTTTCGGTGGCCTCTTGCCGGAAGAAGGCCAGCGCGACGCTGTGGCCCAGGCGCTCGGC
GTTTCACGGGCCAATGATTTTGCCCTTCTCGATCGTCTCGGTGGCGATGTCGCGGGCGCGCTCCAGCTTCTGCCGCCCGG
CGAACCACAGATGGGTCTTGCCGACGAGCGGCAGGCGAGCCCGCTCGATGATGCGGCCCTGATCCGGGTACTGGAGGCGC
TGCCCCTTCGCCCGCTGCTGGCGGGCGAGCGCGGACTGCGGCTCTCGCTGGCCGGTGCGCAGTCGAAGGTGCCGGTCGTT
CTGGTGGATGGCGCGGTGGCGTTGCCTGTGCCGGGCCAGCCGACGACGCATATTCTCAAACCGCCGATCTCGCGCTTCGC
GGCCACGACCGAGAACGAGGCCTTTGTGATGTGCCTTGCCGCGGCAATCGGTCTCGATGTCGCGCCGGTAGAGGCGCGTA
TCGTGCAGGGTCGCCCATTTCTGCTGGTCCAGCGCTACGACCGCGACATCGGCGGTGATGGGGCTGTGCACCGGATCCAT
CAGGAAGATTTCTGCCAGGCGCTCGGTGTACCACCGGAGATCAAATATGCCAGCGAAGGTGGCCCGACCTTCAAGGATTG
CTTCGCGCTGCTCCGCCGTGTCGCTGCCAGGCCCGCCGTCGATGTGCTGAAGCTTCTCGACGCGGTGATCTTCAATGTGA
TCGCCGGGAATGCCGATGCGCACGGCAAGAACTTCTCGATCCTCTATGACACCGGAGGCCCGCGCCTCGCCCCGCTCTAC
GACCTGCTGGCGACTGTCGCCTATCCCGACCTCTCGCCGAATTTCGCCATGAAGATCGGGAAACGGGCGACGCTCGCCGA
GCTGGATGCCAAGGGCTGGGCAACCTTCGCAGCTGAGACGGGTCTCGGCCTGCCGCTGATCCGGCGACGGGTTGCGGAGA
TCAGCAAGGGCGTGCTTGAGAAGGCAACCGCCGTTGCAGAGGAGCTTTCGCCGTCCGGCTTGGACCGACGGGCCAGTGAG
CAGTTTGCCGACATGATCCGTGACCGTGCCGAGCGTTGTGCCCTGACGGTCGGGGGGAGGGCCCGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8298.68 Da
• Isoelectric Point: 10.3067

>AT27879 WP_014747679.1 NC_017957:c672684-672442 [Tistrella mobilis KA081020-065]
MARRTLTPAEIGDIVRATRKAAGLRQDELAGAAGVGLRFIVDLEAGKPTAQIGKTLQVLAALGCSFDIMPPPDINGGRKA

Nucleotide

• Length: 243 bp

>AT27879 NC_017957:c672684-672442 [Tistrella mobilis KA081020-065]
ATGGCCAGACGCACGCTCACCCCTGCCGAGATCGGCGACATTGTCCGCGCCACCCGCAAGGCCGCAGGCCTGCGGCAGGA
CGAGCTTGCGGGCGCAGCCGGGGTCGGCTTGCGCTTCATCGTCGATCTCGAAGCGGGCAAGCCGACCGCCCAGATCGGCA
AGACGCTTCAGGTCCTGGCGGCCCTTGGATGTTCGTTCGACATCATGCCGCCGCCGGATATCAACGGAGGGCGGAAGGCA
TGA

Structures

Toxin

• AlphaFold DB: I3TTB3

Antitoxin

• AlphaFold DB: I3TTB4