Detailed information of TA system TA027876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 4675..5203
• Replicon: plasmid pTM1
• Accession: NC_017957
• Organism: Tistrella mobilis KA081020-065

Toxin (Protein)

• Gene name: parE
• Uniprot ID: I3TRM0
• Locus tag: TMO_RS17980
• Protein ID: WP_014747085.1
• Coordinates: 4910..5203 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: I3TRL9
• Locus tag: TMO_RS17975
• Protein ID: WP_014747084.1
• Coordinates: 4675..4920 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMO_RS31725	1..3501	+	3501	WP_014747081.1	AMP-binding protein	-
TMO_RS17965	3566..4135	+	570	WP_014747082.1	hypothetical protein	-
TMO_RS17970	4321..4569	+	249	Protein_2	antitoxin of toxin-antitoxin stability system	-
TMO_RS17975	4675..4920	+	246	WP_014747084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
TMO_RS17980	4910..5203	+	294	WP_014747085.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TMO_RS29695	5712..7493	+	1782	WP_051045541.1	methyltransferase domain-containing protein	-
TMO_RS31730	7712..7870	+	159	WP_014747087.1	hypothetical protein	-
TMO_RS31735	8237..8401	+	165	WP_187297851.1	hypothetical protein	-
TMO_RS17990	8487..9398	+	912	WP_041606861.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..692456	692456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10719.47 Da
• Isoelectric Point: 10.5335

>T27876 WP_014747085.1 NC_017957:4910-5203 [Tistrella mobilis KA081020-065]
MAAERPVRVSPRAEADLENIFAHTTARWGIQQAQTYHAGFIAAFRNLAAGRAGVMPAVRTGYLKLRVGMHVIYLKEGPAT
IDVIRILHAAMDVDRHL

Nucleotide

• Length: 294 bp

>T27876 NC_017957:4910-5203 [Tistrella mobilis KA081020-065]
ATGGCGGCTGAACGGCCGGTCCGGGTCAGCCCCCGGGCCGAAGCCGATCTGGAGAACATTTTTGCCCACACCACCGCCCG
CTGGGGCATCCAACAAGCGCAGACATACCACGCCGGTTTCATCGCAGCGTTCCGGAACCTGGCAGCCGGGCGCGCCGGCG
TGATGCCGGCCGTGCGCACCGGCTATCTGAAGCTGCGGGTGGGCATGCACGTGATCTATCTGAAAGAGGGGCCGGCAACG
ATCGATGTCATCCGCATTCTGCATGCTGCAATGGATGTCGATCGGCATTTGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8925.91 Da
• Isoelectric Point: 5.5235

>AT27876 WP_014747084.1 NC_017957:4675-4920 [Tistrella mobilis KA081020-065]
MARTTSITLGDHFADFVQTQVATGRYGSTSDVVRAGLRLLEEHEARVRALHDALQAGEDSGEPRLWDDEAFLARMMARHG
G

Nucleotide

• Length: 246 bp

>AT27876 NC_017957:4675-4920 [Tistrella mobilis KA081020-065]
ATGGCGCGAACCACCTCAATCACCCTCGGCGATCACTTTGCCGATTTCGTCCAAACGCAGGTGGCGACCGGCCGCTATGG
CTCGACCAGTGACGTCGTCCGCGCCGGACTGCGTCTTCTTGAAGAGCACGAGGCGCGCGTCCGCGCGCTTCATGATGCCC
TGCAGGCGGGTGAAGACTCGGGTGAACCGCGCCTCTGGGATGACGAAGCCTTCCTGGCGCGGATGATGGCCCGACATGGC
GGCTGA

Structures

Toxin

• AlphaFold DB: I3TRM0

Antitoxin

• AlphaFold DB: I3TRL9