Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4718994..4719491 Replicon chromosome
Accession NZ_CP123240
Organism Escherichia coli strain YZLc1-3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N4725_RS22955 Protein ID WP_001173343.1
Coordinates 4718994..4719167 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N4725_RS22960 Protein ID WP_000398619.1
Coordinates 4719195..4719491 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4725_RS22945 (4715664) 4715664..4717121 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
N4725_RS22950 (4717358) 4717358..4718875 + 1518 WP_001550472.1 lysine--tRNA ligase -
N4725_RS22955 (4718994) 4718994..4719167 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N4725_RS22960 (4719195) 4719195..4719491 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N4725_RS22965 (4719720) 4719720..4719992 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N4725_RS22970 (4720004) 4720004..4720234 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
N4725_RS22975 (4720414) 4720414..4722045 + 1632 WP_001216477.1 sensor histidine kinase -
N4725_RS22980 (4722042) 4722042..4722761 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 papB / papI 4645071..4719491 74420


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T278748 WP_001173343.1 NZ_CP123240:c4719167-4718994 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT278748 WP_000398619.1 NZ_CP123240:c4719491-4719195 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References