Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4363861..4364437 Replicon chromosome
Accession NZ_CP123240
Organism Escherichia coli strain YZLc1-3

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag N4725_RS21175 Protein ID WP_001297643.1
Coordinates 4364150..4364437 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag N4725_RS21170 Protein ID WP_000063160.1
Coordinates 4363861..4364163 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4725_RS21155 (4360457) 4360457..4362607 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
N4725_RS21160 (4362657) 4362657..4362860 + 204 WP_000467859.1 YbdD/YjiX family protein -
N4725_RS21165 (4362871) 4362871..4363827 + 957 WP_001297640.1 GTPase -
N4725_RS21170 (4363861) 4363861..4364163 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
N4725_RS21175 (4364150) 4364150..4364437 - 288 WP_001297643.1 BrnT family toxin Toxin
N4725_RS21180 (4364636) 4364636..4365332 - 697 Protein_4144 IS1 family transposase -
N4725_RS21185 (4365647) 4365647..4366879 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
N4725_RS21190 (4366920) 4366920..4368200 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4364636..4364845 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T278744 WP_001297643.1 NZ_CP123240:c4364437-4364150 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT278744 WP_000063160.1 NZ_CP123240:c4364163-4363861 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References