Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 22049..22596 Replicon plasmid pPae2250X
Accession NZ_CP123223
Organism Pseudomonas amygdali pv. aesculi strain 2250

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag QEP70_RS28335 Protein ID WP_003319696.1
Coordinates 22270..22596 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag QEP70_RS28330 Protein ID WP_003319697.1
Coordinates 22049..22273 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEP70_RS28290 17530..18831 + 1302 WP_060406807.1 replication initiation protein -
QEP70_RS28295 18991..19407 + 417 WP_010214328.1 hypothetical protein -
QEP70_RS28300 19443..19673 + 231 WP_010214330.1 hypothetical protein -
QEP70_RS28305 19751..20302 + 552 WP_243580961.1 hypothetical protein -
QEP70_RS28310 20370..20975 - 606 WP_010214332.1 hypothetical protein -
QEP70_RS28315 20975..21148 - 174 WP_005759896.1 hypothetical protein -
QEP70_RS28320 21331..21522 - 192 WP_060406806.1 hypothetical protein -
QEP70_RS28325 21572..21811 - 240 Protein_25 recombinase family protein -
QEP70_RS28330 22049..22273 + 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
QEP70_RS28335 22270..22596 + 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QEP70_RS28340 22979..23382 - 404 Protein_28 transposase -
QEP70_RS28345 23540..25909 + 2370 WP_010213983.1 hypothetical protein -
QEP70_RS28350 25911..26117 + 207 WP_031599323.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49726 49726
- flank IS/Tn - - 23059..23331 272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T278662 WP_003319696.1 NZ_CP123223:22270-22596 [Pseudomonas amygdali pv. aesculi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT278662 WP_003319697.1 NZ_CP123223:22049-22273 [Pseudomonas amygdali pv. aesculi]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References