Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 29849..30396 Replicon plasmid pPae2250P
Accession NZ_CP123222
Organism Pseudomonas amygdali pv. aesculi strain 2250

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag QEP70_RS27975 Protein ID WP_003319696.1
Coordinates 30070..30396 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag QEP70_RS27970 Protein ID WP_003319697.1
Coordinates 29849..30073 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEP70_RS27945 24887..25168 + 282 WP_243580957.1 hypothetical protein -
QEP70_RS27950 25149..26382 + 1234 WP_280124520.1 IS91 family transposase -
QEP70_RS27955 26652..27881 + 1230 Protein_25 IS91 family transposase -
QEP70_RS27960 28232..28555 - 324 WP_005734861.1 hypothetical protein -
QEP70_RS27965 28548..29246 - 699 WP_005734862.1 ParA family protein -
QEP70_RS27970 29849..30073 + 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
QEP70_RS27975 30070..30396 + 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QEP70_RS27980 30608..32008 - 1401 WP_010217001.1 protein-tyrosine phosphatase family protein -
QEP70_RS27985 32213..33424 + 1212 WP_234785911.1 IS91 family transposase -
QEP70_RS27990 34294..34668 - 375 WP_234785910.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..70372 70372
- inside IScluster/Tn - - 217..55047 54830


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T278661 WP_003319696.1 NZ_CP123222:30070-30396 [Pseudomonas amygdali pv. aesculi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT278661 WP_003319697.1 NZ_CP123222:29849-30073 [Pseudomonas amygdali pv. aesculi]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References