Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4520..5067 Replicon plasmid pPae2203X
Accession NZ_CP123220
Organism Pseudomonas amygdali pv. aesculi strain 2203

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag QEP68_RS29955 Protein ID WP_003319696.1
Coordinates 4741..5067 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag QEP68_RS29950 Protein ID WP_003319697.1
Coordinates 4520..4744 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEP68_RS29910 1..1302 + 1302 WP_060406807.1 replication initiation protein -
QEP68_RS29915 1462..1878 + 417 WP_010214328.1 hypothetical protein -
QEP68_RS29920 1914..2144 + 231 WP_010214330.1 hypothetical protein -
QEP68_RS29925 2261..2773 + 513 WP_236732330.1 hypothetical protein -
QEP68_RS29930 2841..3446 - 606 WP_010214332.1 hypothetical protein -
QEP68_RS29935 3446..3619 - 174 WP_005759896.1 hypothetical protein -
QEP68_RS29940 3802..3993 - 192 WP_060406806.1 hypothetical protein -
QEP68_RS29945 4043..4282 - 240 Protein_7 recombinase family protein -
QEP68_RS29950 4520..4744 + 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
QEP68_RS29955 4741..5067 + 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QEP68_RS29960 5450..5853 - 404 Protein_10 transposase -
QEP68_RS29965 6011..8380 + 2370 WP_010213983.1 hypothetical protein -
QEP68_RS29970 8382..8588 + 207 WP_031599323.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49730 49730
- flank IS/Tn - - 5530..5802 272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T278659 WP_003319696.1 NZ_CP123220:4741-5067 [Pseudomonas amygdali pv. aesculi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT278659 WP_003319697.1 NZ_CP123220:4520-4744 [Pseudomonas amygdali pv. aesculi]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References