Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 22349..22896 Replicon plasmid pPae2203P
Accession NZ_CP123218
Organism Pseudomonas amygdali pv. aesculi strain 2203

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag QEP68_RS29315 Protein ID WP_003319696.1
Coordinates 22349..22675 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag QEP68_RS29320 Protein ID WP_003319697.1
Coordinates 22672..22896 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEP68_RS29300 18077..18451 + 375 WP_234785910.1 hypothetical protein -
QEP68_RS29305 19321..20532 - 1212 WP_234785911.1 IS91 family transposase -
QEP68_RS29310 21202..22137 + 936 WP_280125002.1 protein-tyrosine phosphatase family protein -
QEP68_RS29315 22349..22675 - 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QEP68_RS29320 22672..22896 - 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
QEP68_RS29325 23499..24197 + 699 WP_005734862.1 ParA family protein -
QEP68_RS29330 24190..24513 + 324 WP_005734861.1 hypothetical protein -
QEP68_RS29335 24864..26093 - 1230 WP_081087346.1 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..70379 70379
- inside IScluster/Tn - - 3390..59900 56510


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T278657 WP_003319696.1 NZ_CP123218:c22675-22349 [Pseudomonas amygdali pv. aesculi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT278657 WP_003319697.1 NZ_CP123218:c22896-22672 [Pseudomonas amygdali pv. aesculi]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References