Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 54122..54669 Replicon plasmid pPae1804P
Accession NZ_CP123211
Organism Pseudomonas amygdali pv. aesculi strain 1804

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag QEP69_RS28215 Protein ID WP_003319696.1
Coordinates 54343..54669 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag QEP69_RS28210 Protein ID WP_003319697.1
Coordinates 54122..54346 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEP69_RS28185 49160..49441 + 282 WP_243580957.1 hypothetical protein -
QEP69_RS28190 49422..50655 + 1234 WP_280124520.1 IS91 family transposase -
QEP69_RS28195 50925..52154 + 1230 WP_243580959.1 IS91 family transposase -
QEP69_RS28200 52505..52828 - 324 WP_005734861.1 hypothetical protein -
QEP69_RS28205 52821..53519 - 699 WP_005734862.1 ParA family protein -
QEP69_RS28210 54122..54346 + 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
QEP69_RS28215 54343..54669 + 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QEP69_RS28220 54881..56281 - 1401 WP_010217001.1 protein-tyrosine phosphatase family protein -
QEP69_RS28225 56486..57697 + 1212 WP_234785911.1 IS91 family transposase -
QEP69_RS28230 58567..58941 - 375 WP_234785910.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..70047 70047
- inside IScluster/Tn - - 17454..69929 52475


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T278645 WP_003319696.1 NZ_CP123211:54343-54669 [Pseudomonas amygdali pv. aesculi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT278645 WP_003319697.1 NZ_CP123211:54122-54346 [Pseudomonas amygdali pv. aesculi]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References