Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 42197..42744 Replicon plasmid pPae1804X
Accession NZ_CP123210
Organism Pseudomonas amygdali pv. aesculi strain 1804

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag QEP69_RS27910 Protein ID WP_003319696.1
Coordinates 42418..42744 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag QEP69_RS27905 Protein ID WP_003319697.1
Coordinates 42197..42421 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEP69_RS27865 37678..38979 + 1302 WP_060406807.1 replication initiation protein -
QEP69_RS27870 39139..39555 + 417 WP_010214328.1 hypothetical protein -
QEP69_RS27875 39591..39821 + 231 WP_010214330.1 hypothetical protein -
QEP69_RS27880 39866..40450 + 585 WP_280124748.1 hypothetical protein -
QEP69_RS27885 40518..41123 - 606 WP_010214332.1 hypothetical protein -
QEP69_RS27890 41123..41296 - 174 WP_005759896.1 hypothetical protein -
QEP69_RS27895 41479..41670 - 192 WP_060406806.1 hypothetical protein -
QEP69_RS27900 41720..41959 - 240 Protein_46 recombinase family protein -
QEP69_RS27905 42197..42421 + 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
QEP69_RS27910 42418..42744 + 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QEP69_RS27915 43127..43530 - 404 Protein_49 transposase -
QEP69_RS27920 43688..46057 + 2370 WP_010213983.1 hypothetical protein -
QEP69_RS27925 46059..46265 + 207 WP_031599323.1 hypothetical protein -
QEP69_RS27930 46426..46599 - 174 WP_010213980.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49732 49732
- flank IS/Tn - - 43207..43479 272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T278643 WP_003319696.1 NZ_CP123210:42418-42744 [Pseudomonas amygdali pv. aesculi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT278643 WP_003319697.1 NZ_CP123210:42197-42421 [Pseudomonas amygdali pv. aesculi]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References