278501

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2886542..2888262 Replicon chromosome
Accession NZ_CP123029
Organism Escherichia coli strain 20-20

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag QEN57_RS14040 Protein ID WP_000312833.1
Coordinates 2886542..2887198 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag QEN57_RS14045 Protein ID WP_000634203.1
Coordinates 2887195..2888262 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN57_RS14025 (2882457) 2882457..2882696 + 240 WP_024224214.1 hypothetical protein -
QEN57_RS14030 (2882736) 2882736..2885174 + 2439 WP_032180025.1 type I restriction-modification system subunit M -
QEN57_RS14035 (2885174) 2885174..2886529 + 1356 WP_223349791.1 restriction endonuclease subunit S -
QEN57_RS14040 (2886542) 2886542..2887198 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
QEN57_RS14045 (2887195) 2887195..2888262 + 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
QEN57_RS14050 (2888281) 2888281..2891376 + 3096 WP_000108736.1 HsdR family type I site-specific deoxyribonuclease -
QEN57_RS14055 (2891376) 2891376..2892092 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
QEN57_RS14060 (2892437) 2892437..2893051 + 615 Protein_2756 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage blaCMY-2 - 2869670..2914365 44695


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T278501 WP_000312833.1 NZ_CP123029:2886542-2887198 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT278501 WP_000634203.1 NZ_CP123029:2887195-2888262 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References