Detailed information of TA system TA027848

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 747281..747944
• Replicon: chromosome
• Accession: NC_017933
• Organism: Cronobacter sakazakii ES15

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: ES15_RS03555
• Protein ID: WP_014728113.1
• Coordinates: 747528..747944 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A3Q9CFW7
• Locus tag: ES15_RS03550
• Protein ID: WP_007870701.1
• Coordinates: 747281..747547 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES15_RS03525	742309..743742	-	1434	WP_014728110.1	6-phospho-beta-glucosidase	-
ES15_RS03530	743894..744622	-	729	WP_041460817.1	MurR/RpiR family transcriptional regulator	-
ES15_RS03535	744799..745113	+	315	WP_014728112.1	N(4)-acetylcytidine aminohydrolase	-
ES15_RS03540	745256..745909	+	654	WP_007777939.1	hemolysin III family protein	-
ES15_RS03545	746014..747003	-	990	WP_007870703.1	tRNA-modifying protein YgfZ	-
ES15_RS03550	747281..747547	+	267	WP_007870701.1	FAD assembly factor SdhE	Antitoxin
ES15_RS03555	747528..747944	+	417	WP_014728113.1	protein YgfX	Toxin
ES15_RS03560	747958..748479	-	522	WP_004385676.1	flavodoxin FldB	-
ES15_RS03565	748581..749477	+	897	WP_041460818.1	site-specific tyrosine recombinase XerD	-
ES15_RS03570	749500..750219	+	720	WP_004385678.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
ES15_RS03575	750223..751956	+	1734	WP_007870697.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16450.46 Da
• Isoelectric Point: 11.4887

>T27848 WP_014728113.1 NC_017933:747528-747944 [Cronobacter sakazakii ES15]
VVLWHSDLRVSWRSQWLSLLLHGAVAVIILLLPWPLRYLPVWMLLLSMVVFDCVRSQRRINAFQGEVSLTADYQLRWQGV
DWQICATPWMLRSGMMLRLRHPKTTRRHHVWLAADSMTEAEWRDLRRLLLQQPVGDKR

Nucleotide

• Length: 417 bp

>T27848 NC_017933:747528-747944 [Cronobacter sakazakii ES15]
GTGGTCCTGTGGCACTCTGATCTTCGCGTTTCATGGCGCTCGCAGTGGCTATCGCTGCTGCTGCACGGCGCCGTGGCCGT
TATTATTTTGCTGCTTCCCTGGCCGCTGCGGTATCTGCCCGTGTGGATGCTGTTGCTGTCTATGGTGGTGTTTGACTGCG
TGCGTAGCCAACGGCGTATCAATGCGTTCCAGGGCGAGGTCAGCCTGACGGCTGATTATCAGCTACGCTGGCAGGGCGTT
GACTGGCAGATTTGCGCCACGCCCTGGATGTTGCGCAGCGGAATGATGCTGCGGCTGCGTCATCCGAAAACGACGCGCCG
CCATCATGTCTGGCTGGCGGCGGATAGCATGACGGAAGCGGAGTGGCGCGATTTGCGTCGTCTTCTGCTGCAACAGCCCG
TAGGCGACAAACGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.89 Da
• Isoelectric Point: 4.8310

>AT27848 WP_007870701.1 NC_017933:747281-747547 [Cronobacter sakazakii ES15]
MDINNKARIHWACRRGMRELDISIMPFFEYDYDSLSDEDKRVFVRLLECDDPDLFNWLMNHGKPADAELARMVELIQQRN
QARGPVAL

Nucleotide

• Length: 267 bp

>AT27848 NC_017933:747281-747547 [Cronobacter sakazakii ES15]
ATGGATATTAATAATAAAGCCCGTATTCACTGGGCATGTCGCAGAGGTATGCGCGAGCTGGATATTTCCATCATGCCTTT
CTTTGAATATGACTATGACTCCCTGAGCGACGAAGACAAACGCGTTTTTGTCCGCCTGCTGGAGTGTGACGATCCTGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCAGCTGATGCGGAGCTGGCGCGAATGGTGGAATTAATCCAGCAGCGGAAT
CAGGCACGTGGTCCTGTGGCACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q9CFW7