278476

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2909622..2911342 Replicon chromosome
Accession NZ_CP123024
Organism Escherichia coli strain 20-16

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag QEN53_RS14190 Protein ID WP_000312833.1
Coordinates 2909622..2910278 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag QEN53_RS14195 Protein ID WP_000634203.1
Coordinates 2910275..2911342 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN53_RS14175 (2905537) 2905537..2905776 + 240 WP_024224214.1 hypothetical protein -
QEN53_RS14180 (2905816) 2905816..2908254 + 2439 WP_032180025.1 type I restriction-modification system subunit M -
QEN53_RS14185 (2908254) 2908254..2909609 + 1356 WP_223349791.1 restriction endonuclease subunit S -
QEN53_RS14190 (2909622) 2909622..2910278 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
QEN53_RS14195 (2910275) 2910275..2911342 + 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
QEN53_RS14200 (2911361) 2911361..2914456 + 3096 WP_000108736.1 HsdR family type I site-specific deoxyribonuclease -
QEN53_RS14205 (2914456) 2914456..2915172 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
QEN53_RS14210 (2915517) 2915517..2916131 + 615 Protein_2787 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage blaCMY-2 - 2902184..2932463 30279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T278476 WP_000312833.1 NZ_CP123024:2909622-2910278 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT278476 WP_000634203.1 NZ_CP123024:2910275-2911342 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References