Detailed information of TA system TA027846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 407263..407933
• Replicon: chromosome
• Accession: NC_017933
• Organism: Cronobacter sakazakii ES15

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: ES15_RS01945
• Protein ID: WP_007852532.1
• Coordinates: 407263..407613 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: ES15_RS01950
• Protein ID: WP_014727946.1
• Coordinates: 407610..407933 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES15_RS01930	404813..406444	+	1632	WP_007891561.1	Na/Pi cotransporter family protein	-
ES15_RS01935	406461..406736	-	276	WP_007852536.1	putative addiction module antidote protein	-
ES15_RS01940	406741..407046	-	306	WP_014727945.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ES15_RS01945	407263..407613	+	351	WP_007852532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ES15_RS01950	407610..407933	+	324	WP_014727946.1	helix-turn-helix domain-containing protein	Antitoxin
ES15_RS01955	407964..408236	-	273	WP_007702377.1	DUF3811 domain-containing protein	-
ES15_RS01960	408328..409263	-	936	WP_004387518.1	ketopantoate/pantoate/pantothenate transporter PanS	-
ES15_RS01965	409425..411017	-	1593	WP_014727947.1	RNA repair transcriptional activator RtcR	-
ES15_RS01975	411494..412627	+	1134	WP_014727949.1	slipin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	406461..414658	8197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.99 Da
• Isoelectric Point: 10.2932

>T27846 WP_007852532.1 NC_017933:407263-407613 [Cronobacter sakazakii ES15]
MIKPLFWVGQARKDLQALPEDVQDLFGYALYLAQQGRRHPQARPLKGFGGAGVLEVVEDYQGNAFRAVYTVRLGEAIYVL
HVFQKKSSSGIATPRPEMEKIEQRLKAAQRHAGGLK

Nucleotide

• Length: 351 bp

>T27846 NC_017933:407263-407613 [Cronobacter sakazakii ES15]
ATGATAAAACCGTTGTTTTGGGTCGGGCAGGCCAGAAAAGATCTTCAGGCGCTGCCGGAGGATGTACAGGATCTTTTTGG
CTATGCACTTTATCTGGCGCAGCAGGGCAGACGCCATCCGCAGGCCAGGCCGCTTAAAGGTTTTGGCGGCGCGGGTGTGC
TTGAGGTCGTGGAGGATTATCAGGGGAATGCCTTTCGGGCGGTCTATACCGTTCGTCTGGGCGAGGCGATTTATGTCCTG
CATGTATTCCAGAAAAAATCCTCTTCCGGGATTGCCACGCCCCGCCCGGAGATGGAAAAAATTGAACAGCGACTTAAAGC
CGCGCAACGCCACGCCGGAGGGCTGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11794.88 Da
• Isoelectric Point: 7.9747

>AT27846 WP_014727946.1 NC_017933:407610-407933 [Cronobacter sakazakii ES15]
MTADIERSNGNVYDDLGLIDAEEMQLKAQLAMAISDILKARGLTQQEAARLLGMTQPKLSLLLRGQFRGISETKMMACLM
LLGRDISIVVGEAQQARGKMRLIYQPA

Nucleotide

• Length: 324 bp

>AT27846 NC_017933:407610-407933 [Cronobacter sakazakii ES15]
ATGACAGCAGACATTGAACGCAGTAACGGAAATGTGTATGACGATCTCGGGCTTATCGACGCCGAAGAGATGCAGCTAAA
AGCGCAGCTCGCTATGGCCATTAGCGACATTCTTAAAGCGCGCGGTTTAACCCAACAGGAGGCGGCCCGTCTTCTGGGAA
TGACCCAGCCAAAACTCTCTTTATTACTTCGCGGCCAGTTTCGCGGGATCAGCGAAACGAAAATGATGGCCTGCCTGATG
CTCCTCGGGCGGGATATCAGCATCGTCGTGGGCGAGGCACAACAGGCGCGCGGAAAAATGCGCCTGATCTATCAGCCCGC
CTGA