Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1962142..1962725 Replicon chromosome
Accession NZ_CP123009
Organism Escherichia coli O155 strain NWU_3

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag QEN28_RS09610 Protein ID WP_000254745.1
Coordinates 1962390..1962725 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QEN28_RS09605 Protein ID WP_000581937.1
Coordinates 1962142..1962390 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN28_RS09595 (1958481) 1958481..1959782 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QEN28_RS09600 (1959830) 1959830..1962064 + 2235 WP_000226815.1 GTP pyrophosphokinase -
QEN28_RS09605 (1962142) 1962142..1962390 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QEN28_RS09610 (1962390) 1962390..1962725 + 336 WP_000254745.1 endoribonuclease MazF Toxin
QEN28_RS09615 (1962796) 1962796..1963587 + 792 WP_136721162.1 nucleoside triphosphate pyrophosphohydrolase -
QEN28_RS09620 (1963815) 1963815..1965452 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QEN28_RS09625 (1965540) 1965540..1966838 + 1299 WP_000036723.1 phosphopyruvate hydratase -
QEN28_RS09630 (1966894) 1966894..1967256 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T278394 WP_000254745.1 NZ_CP123009:1962390-1962725 [Escherichia coli O155]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT278394 WP_000581937.1 NZ_CP123009:1962142-1962390 [Escherichia coli O155]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References